Authors:S.ibel Başkurt, E. Doğaç, V. Taşkın, and Belg.in Taşkın
In the present study, the frequencies of three organophosphate (OP) resistance-associated mutations in acetylcholinesterase gene of Bactrocera oleae (BoAce) populations collected from 8 different important olivegrowing areas in the west part of Turkey were determined. Populations were sampled from the areas that have been treated with only the pyrethroid α-cypermethrin; pyrethroids plus OPs; deltamethrin with pheromone eco-traps, and no insecticide treatment applied areas for many years. For Ile214Val and Gly488Ser point mutations PCR-RFLP and for Δ3Q deletion mutation PCR diagnostic tests were carried out. Seventy-two percent of the total individuals analyzed in the study were exhibited heterozygous genotype (RS) for both Ile214Val and Gly488Ser point and homozygous susceptible genotype (SS) for Δ3Q deletion mutations. This RS/RS/SS combination together with RS/RR/SS with the frequency of 13% were the most common two combinations observed in all of the populations under different insecticide regimes, even in the populations under no insecticide pressure for many years. Independent evaluation of the three mutations resulted in 0.450, 0.534 and 0.037 frequency values for the resistant alleles of 214Val, 488Ser and Δ3Q mutations, respectively. Among the studied populations, the frequencies of resistant alleles for the positions of 214 and 488 were not differed from each other. However, in 3 of the populations the frequency of the R allele of Δ3Q was zero and it changed between 0.025 and 0.100 in the remaining five populations. Results of this study contributed to the distribution pattern of the two point mutations in Europe and a pattern for Δ3Q mutation was determined for the first time in the field collected olive fly samples.
Authors:J. Kundu, J. Jarošová, S. Gadiou, and G. Cervená
One-step RT-PCR followed by a RFLP assay was developed for the typing of
Barley yellow dwarf virus
(BYDV) member of
genera. RFLP analysis performed on 23 samples including one lab isolate showed three types of
II restriction profile. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. One-step RT-PCR together with RFLP presents an easy and reliable assay for routine BYDV typing. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic.
Authors:Á. Maróti-Agóts, I. Bodó, L. Jávorka, Alice Gyurmán, N. Solymosi, Petra Zenke, Marita Skogseth, and L. Zöldág
The synthesis of Heat Shock Protein 70.2 mRNA is also regulated by the Upper Promoter elements of the gene. This promoter region is polymorphic in cattle. These polymorphisms have a major effect on the activity of the mRNA transcription. In a comparison of quantity of transcribed mRNA from the wild type and AP2 mutant allele the wild type can produce 2–3-fold more transcripts.The Hungarian Grey Cattle (HG) and Norwegian Red (NFR) as control breed were genotyped with PCR-RFLP method. Our results showed that the frequencies of alleles in breeds (p(wt)HG = 0.859419, p(wt)NFR = 0.5) are different. The effective response to heat stress in the Norwegian Red seems to be less important than in the Hungarian Grey breed. The extensive keeping in hot and arid region during centuries could have been proved as selection pressure for the heat tolerance.Our results combined with the global climate forecasts emphasize the role of autochthonous, well adopted, heat tolerant breeds in the near future.
. E. (2003) PCR-RFLP is used to investigate relations among species in the entomopathogenic genera Eryniopsis and Entomophaga. Mycologia 95 , 262-268.
PCR-RFLP is used to investigate relations among species in the
Authors:Sulejman Redžepović, Sanja Sikora, Josip Čolo, Mihaela Blažinkov, and Marija Pecina
Sikora S. — Redžepović S. 2003. Genotypic characterization of indigenous soybean rhizobia by PCR-RFLP of 16S rDNA, rep-PCR, rep-PCR and RAPD analysis. Food Technology and Biotechnology vol. 41 no. 1 61
Authors:Z. Jurković, K. Dugalić, M. Viljevac, I. Piližota, A. Vokurka, B. Puškar, and I. Pejić
Sertkaya, G., Ulubas, C., Caglayan, K. (2003): Detection and characterisation of Plum pox potyvirus (PPV) by DAS-ELISA and RT-PCR/RFLP analysis in Turkey.
Turk J. Agric. For.