Authors:Ángela Lacombe-Antoneli, Segundo Píriz, Alberto Quesada, and Santiago Vadillo
Footrot is widely considered the most severe and most common foot pathology in small ruminants. This study tested the ability of a molecular typing system based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay of the 16S rRNA gene to discriminate between the strict anaerobe genera most commonly isolated from footrot (
Bacteroides, Dichelobacter, Fusobacterium, Porphyromonas
) in goats in Extremadura (Spain), with a view to facilitating identification for diagnostic purposes and thus providing a useful tool for future epidemiological studies. Although the electrophoretic patterns obtained with the enzyme
1I were more readily interpreted, and may thus be the best initial option, results may be confirmed by a second enzyme (
I). The PCR-RFLP assay of the 16S rRNA gene may therefore prove a useful addition to conventional biochemical identification techniques, providing taxonomic information at genus level.
Furlanello , T. (2008) : Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection bymeans of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease . Vet. Parasitol . 157 , 211 – 221
Authors:Judit Bíró, Noémi Erdei, Ibolya Székely, and L. Stipkovits
Increasing use of Mycoplasma gallisepticum (MG) live vaccines has led to a need for the differentiation of MG strains. The MG strains MK-7, MS-16, S6, FS-9 and R strains and the MG live vaccine strain F were compared by random amplification of polymorphic DNA (RAPD) in this study. Using RAPD, different patterns were found among the MG strains. In addition to this, we examined the differentiating potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) primers targeted at the crmA, crmB, crmC, gapA, mgc2 and pvpA genes encoding cytadherence-related surface proteins. These proteins may take part in the pathogenesis of MG-induced disease. Differentiation of strain F is based on the identification of restriction enzyme sites in the PCR amplicons. Using HphI enzyme, crmC PCR amplicons produced different RFLP patterns. Digestion of amplicons of gapA-specific PCR with MboI enzyme also produced distinct patterns. Differences were observed among strains R and F by digestion of mgc2 PCR amplicons with HaeIII and VspI enzymes and digestion of pvpA PCR amplicons with AccI, PvuII and ScrFI endonucleases. This method can be used for the rapid differentiation of vaccine strain from wild strains. Differentiation of MG strains is a great advantage for diagnosticians or practitioners and it is useful for epidemiological studies.
Authors:I. Anton, A. Zsolnai, I. Komlósi, A. Király, and L. Fésüs
The effect of the porcine myogenin (Myog) 3' polymorphism on birth weight, growth rate, carcass weight, lean weight, lean meat percentage and backfat thickness has been investigated in Hungarian Large White pigs. MYOG genotypes were determined by PCR-RFLP assay. The obtained MYOGA frequency value was 0.6275. Due to the small number of BB piglets the effect of the MYOG genotypes on birth weight was not significant; however, an increasing tendency was observed from genotype AA to BB. The growth rate difference between MYOG genotypes was significant: BB animals showed the highest growth rate values during the fattening period. Since few results are available on the possible use of MYOG gene polymorphism in selection to improve carcass and growth traits, by this study the authors hope to provide additional data on this particular subject.
Leung, E. T. Y., Kam, K. M., Chiu, A., Leung, P. H., Seto, W. H., Yuen, K. Y., Yam, W. C.: Detection of KatGSer315Thr substitution in respiratory specimens from patients with isoniazid-resistant
using PCR-RFLP. J. Med
Authors:István Anton, Katalin Kovács, László Fésüs, József Várhegyi, László Lehel, Zoltán Hajda, J. Polgár, Ferenc Szabó, and Attila Zsolnai
The objective of this study was to estimate the effect of the thyroglobulin (TG) locus on beef quality traits in some beef cattle breeds and to investigate the effect of the DGAT1 locus on milk production traits in the Hungarian Holstein Friesian population. TG and DGAT1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. At the TG locus TT bulls showed the highest fat percentage values in the longissimus dorsi muscle (m. longissimus dorsi); the difference between CC and TT genotypes was significant. DGAT1 GC/GC cows had the highest milk, fat and protein yield values. Due to the relatively small number of GC/GC cows the difference proved to be significant only between AA/AA and AA/GC genotypes.
Authors:M. Arjomandzadegan, P. Owlia, R. Ranjbar, A. Farazi, Masume Sofian, Maryam Sadrnia, E. Ghaznavi-Rad, Larisa Surkova, and L. Titov
., Kwok-Yung, Y., Wing-Cheong, Y.: Detection of KatG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP. J. Med. Microbiol. 52 , 999–1003 (2003