Authors:J. Kvicala, V. Zamrazil, J. Nemecek, and J. Jiranek
To estimate the status of selenium in seniors of the South Bohemia region, Czech Republic, 481 serum samples from seniors
living in 17 Asylum Houses for seniors in the age between 60 and 99 years were obtained. Samples were analyzed by instrumental
neutron activation analysis with concurrent analysis of 4 reference materials for quality assurance. Average concentration
of serum Se, arithmetic means in individual Asylum Houses, frequency distribution of serum Se concentrations as well as five
years running monitoring of serum Se concentrations of one group of seniors proved selenium deficiency of the elderly population
of the region South Bohemia.
Authors:J. Kvicala, P. Hrdá, V. Zamrazil, J. Nemecek, M. Hill, and V. Jiranek
Selenium is an essential component of selenoproteins, enzymes with extensive regulatory and protective effect in organism.
Immunological effects of Se are documented and are distinct even above concentrations necessary for maximal activity of selenoenzymes.
Therefore, we investigated effect of supplementation by 100 μg of yeast-bound Se on concentrations of thyroid autoantibodies
TPOAb and TgAb in the group of 253 seniors living in the Asylum Houses of South Bohemia. Increase of serum selenium from 59
to 150 μg Se/L serum in supplemented group and from 59 to 72 μg Se/L serum in group with placebo were detected by Instrumental
Neutron Activation Analysis (INAA) and proved increased Se intake during the trial. Autoantibodies were analyzed by ELISA
at the beginning of the trial and after 1 year. Statistical evaluation of results in whole groups (regardless of increased
autoantibodies) by ANOVA manifested significant decrease of TPOAb and TgAb in non-supplemented group while supplementation
did not effect serum autoantibodies concentrations. Evaluation of groups of seniors created from those with increased autoantibodies,
ANOVA demonstrated decrease of TPOAb in both groups but Se supplementation did not affect the decrease. In opposite, TgAb
increased significantly and Se supplementation led to higher increase of TgAb. Recent results of possibility to decrease serum
concentration of TPOAb proved this effect only for high TPOAb concentrations and for higher Se supplements. From this point
of view, it is necessary to conduct subsequent trials with the patients with autoimmune thyreoiditis with different levels
of autoantibodies and detect also serum Se levels.