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Pseudomonads, including P. fluorescens strain MKB 158, can inhibit the development of Fusarium seedling blight disease on wheat and barley. Application of this and other pseudomonads as head sprays inhibits the development of Fusarium head blight disease (FHB) disease on wheat and barley under both field and glasshouse conditions. P. fluorescens strains MKB 158 and MKB 249 also reduced DON contamination of grain under field conditions. Evidence suggests that P. fluorescens does not directly inhibit the growth of Fusarium , but that it potentiates host resistance against this disease. Transcriptome profiling identified barley genes differentially expressed as early events in (a) bacterium-induced resistance to seedling blight and (b) heads following P. fluorescens and Fusarium culmorum co-inoculation. Bacterium-potentiated resistance to Fusarium affects the transcription of many genes that are involved in diverse processes, including cell rescue and defence, metabolism, cell cycle and DNA replication and signalling.

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A multienzyme complex from newly isolated Paenibacillus sp. TW1 was purified from pellet-bound enzyme preparations by elution with 0.25% sucrose and 1.0% triethylamine (TEA), ultrafiltration and Sephacryl S-400 gel filtration chromatography. The purified multienzyme complex showed a single protein band on non-denaturing polyacrylamide gel electrophoresis (native-PAGE). The high molecular mass of the purified multienzyme complex was approximately 1,950 kDa. The complex consisted of xylanase and cellulase activities as the major and minor enzyme subunits, respectively. The complex appeared as at least 18 protein bands on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and as 15 xylanases and 6 cellulases on zymograms. The purified multienzyme complex contained xylanase, α-L-arabinofuranosidase, carboxymethyl cellulase (CMCase), avicelase and cellobiohydrolase. The complex could effectively hydrolyze corn hulls, corncobs and sugarcane bagasse. These results indicate that the multienzyme complex that is produced by this bacterium is a large, novel xylanolytic-cellulolytic enzyme complex.

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Cereal Research Communications
Authors: László Zsiros, Ágnes Szatmári, László Palkovics, Zoltán Klement and Zoltán Bozsó
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Introduction: Escherichia coli and Staphylococcus aureus are important causes of severe diseases like blood stream infections. This study comparatively assessed potential differences in their impact on disease severity in local and systemic infections.

Methods: Over a 5-year interval, patients in whom either E. coli or S. aureus was detected in superficial or primary sterile compartments were assessed for the primary endpoint death during hospital stay and the secondary endpoints duration of hospital stay and infectious disease as the main diagnosis.

Results: Significance was achieved for the impacts as follows: Superficial infection with S. aureus was associated with an odds ratio of 0.27 regarding the risk of death and of 1.42 regarding infectious disease as main diagnosis. Superficial infection with E. coli was associated with a reduced duration of hospital stay by −2.46 days and a reduced odds ratio of infectious diseases as main diagnosis of 0.04. The hospital stay of patients with E. coli was increased due to third-generation cephalosporin and ciprofloxacin resistance, and in the case of patients with S. aureus due to tetracycline and fusidic acid resistance.

Conclusions: Reduced disease severity of superficial infections due to both E. coli and S. aureus and resistance-driven prolonged stays in hospital were confirmed, while other outcome parameters were comparable.

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Biofertilizers are used to improve soil fertility and plant production in sustainable agriculture. However, their applicability depends on several environmental parameters. The aim of our study was to evaluate the effect of free-living bacteria containing fertilizer on the growth of cucumber (Cucumis sativus L. cvs. Delicates) under aluminium (Al) stress. Different responses to Al stress of cucumber growth parameters were examined in terms of root elongation and physiological traits, such as Spad index (relative chlorophyll value), biomass accumulation of root and shoot, Al uptake and selected element contents (Fe, Mn, Zn, Mg) of leaves and root. The applied bacteria containing biofertilizer contains Azotobacter chroococcum and Bacillus megaterium. The dry weights of cucumber shoots and roots decreased in line with the increasing Al concentration. Due to different Al treatments (10−3 M, 10−4 M) higher Al concentration was observed in the leaves, while the amounts of other elements (Fe, Mn, Zn, Mg) decreased. This high Al content of the leaves decreased below the control value when biofertilizer was applied. In the case of the roots the additional biofertilizer treatments compensated the effect of Al. The relative chlorophyll content was reduced during Al-stress in older plants and the biofertilizer moderated this effect. The root/shoot ratio was decreased in all the Al-treatments in comparison to the control. The living bacteria containing fertilizer also had a modifying effect. The root/shoot ratio increased at the 10−4 M Al2(SO4)2 + biofertilizer and 10−4 M Al(NO3)3 + biofertilizer treatments compared to the control and Al-treatments. According to our results the biofertilizer is an alternative nutrient supply for replacing chemical fertilizers because it enhances dry matter production. Biofertilizer usage is also offered under Al polluted environmental conditions. Although, the nutrient solution is a clean system where we can examine the main processes without other effects of natural soils. The soil can modify the results, e.g. the soil-born microorganisms affect nutrient availability, and also can modify the harmful effects of different heavy metals. The understanding of basic processes will help us to know more about the soil behaviour.

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bacterium so far established in Yugoslavia. Plant Protection (Zaš bilja), Belgrade, 219, 57-66 (in Serbian with English summary). Host Plants of the Erwinia amylovora bacterium so far established in Yugoslavia

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Introduction Discovered in 1984, the gastric bacterium Helicobacter pylori has long been known as one of the most successful human pathogens that has infected approximately half of the global population [ 1 ]. Although most

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European Journal of Microbiology and Immunology
Authors: E. Sapi, K. Gupta, K. Wawrzeniak, G. Gaur, J. Torres, K. Filush, A. Melillo and B. Zelger

respiratory pathogen whose infection leads to extra-pulmonary symptoms such as myocarditis, atherosclerosis, reactive arthritis, and nervous system disorders [ 58 , 59 ]. Chlamydia trachomatis is a bacterium responsible for causing sexually transmitted

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