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The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
Bugno, M., Ząbek, T., Golonka, P., Pieńkowska-Schelling, A., Schelling, C and Słota, E. (2008): A case of an intersex horse with 63,X/64,XX/65,XX, del(Y)(q?) karyotype. Cytogenet. Genome Res. 120 , 123
Kolon, T. F., Gray, C. L., Borboroglu, P. G.: Prenatal karyotype and ultrasound discordance in intersex conditions. Urology, 1999, 54 , 1097xvi–1097xix. Borboroglu P. G
Kolon, T. F., Gray, C. L., Borboroglu, P. G.: Prenatal karyotype and ultrasound discordance in intersex conditions. Urology, 1999, 54 , 1097xvi–1097xix. Borboroglu P. G
. F., et al.: Consensus statement on management of intersex disorders. Pediatrics, 2006, 118 (2), e488–e500. Snodgrass, W., Macedo, A., Hoebeke, P., et al.: Hypospadias dilemmas: a round table. J. Pediatr. Urol
