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Abstract
Cytokines and soluble cytokine receptors serve as important protein biomarkers for chronic and infectious disease diagnosis. The development of biosensors capable of detecting cytokines or their soluble receptors in patient bodily fluids is a growing area of research. In an ongoing series of studies to understand the thermal analytical behavior of cytokines and their soluble receptors, dielectric thermal analysis (DETA) and thermogravimetry (TG) were used in investigations to determine if differentiations based on dielectric properties (e.g., conductivity) of the proteins could be identified. Permittivity (∊′) and dielectric loss factor (∊″) measurements were performed over a frequency range of 0.1–300,000 Hz. Up to 20 min, water associated with the samples was conductive, interacting with the proteins and affecting the temperature-dependent relaxation spectra of proteins. A trend analysis revealed differences between surface charge at 0.1 Hz and bulk charge at 300,000 Hz. In addition, the greatest change detected among proteins was due to the conductivity (dielectric loss factor). Beyond a 20 min drying time, the observed conductivity was due to intrinsic properties of the proteins with limited dependence on frequency. A 100% water loss was obtained for samples within 20–30 min by TG. Sample drying by TG could serve as a preparatory step in drying protein samples for further DETA and DSC analysis.
635 646 Schrohl, A. S., Würtz, S., Kohn, E. és mtsai: Banking of biological fluids for studies of disease-associated protein biomarkers. Mol. Cell. Proteomics
Suzuki, M., Wiers, K., Brooks, E. és mtsai: Initial validation of a novel protein biomarker panel for active pediatric lupus nephritis. Pediatr. Res., 2009, 65 , 530–536. Brooks E
198 207 Hewitt SM, Dear J, Star RA: Discovery of protein biomarkers for renal diseases. J. Am. Soc. Nephrol. 15, 1677–1689 (2004) Star
, R. ( 2010 ): Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine . Vet. J. 185 , 23 – 27 . Ercan , N. , Tuzcu , N. , Basbug , O. , Gok
these parameters . Parasit. Vectors 6 , 76 . 10.1186/1756-3305-6-76 Eckersall , P. D. and Bell , R . ( 2010 ): Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine . Vet. J. 185 , 23 – 27 . 10.1016/j
. Fagerquist CK , Bates AH , Heath S , King BC , Garbus BR , Harden LA , et al. Sub-speciating Campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications . J
