Authors:Kitti Sárközi, András Papp, Zsuzsanna Máté, Edina Horváth, Edit Paulik, and Andrea Szabó
Afanasev, I. B., Dorozhko, A. I., Brodskii, A. V., Kostyuk, V. A., Potapovitch, A. I. (1989) Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation
Authors:É. Szőke, G. Petroianu, K. Tekes, B. Benkő, P. Szegi, R. Laufer, and G. Veress
Reversed-phase HPLC has been used to monitor the concentration of the two major Chamomile components rutin and quercetin during rat liver microsomal treatment. The possibility of microsomal oxidative metabolism or stability of these two components was examined using a guard-column without any clean-up. The concentration of quercetin decreased when exposed to rat liver microsomal media whereas the rutin concentration did not change significantly over one hour of treatment.
The thermal behavior of rutin extracted from the buds of Sophora japonica L. by different methods and conditions have been investigated using TG and DTG. The results showed that every sample had
different mass loss, curve shape, and peak location related to varied extraction technology. The TG-DTG characteristics of
the rutin sample extracted by alkali-dissolution and acid-sedimentation with the solution adjusted to pH 9 and simply borax
as stabilizer were highly similar to that of standard rutin, with the maximal purity determined by spectrophotometry. Therefore,
the TG-DTG patterns could be served to characterize rutin extracted from the buds of Sophora japonica L.
An ultrasensitive and rapid method for the determination of epicatechin, rutin, and quercetin was developed using capillary zone electrophoresis with on-line chemiluminescence detection. Under the optimal conditions, the analytes were baseline separated within 12 min. The limits of detection in turn were 0.60 pg mL−1 for epicatechin, 0.50 pg mL−1 for rutin, and 1.0 pg mL−1 for quercetin. The developed method was an easy and reliable method of determining these analytes concentrations in tea, extract Ginkgo biloba, and rutin tablet, demonstrating the feasibility and reliability of the proposed method.
Authors:J. Zhao, L. Zhong, L. Zou, C. Zhang, L. Peng, W. Xiao, and G. Zhao
The purpose of this study was to investigate the effects of endophytic fungi from tartary buckwheat on the host sprout growth and functional metabolite production. Without obvious changes in the appearance of the sprouts, the exogenous fungal mycelia elicitors notably stimulated the sprout growth and rutin accumulation, and the stimulation effect was mainly depended on the mycelia elicitor species along with its treatment dose. Three endophytic fungi Fat6 (Bionectria pityrodes), Fat9 (Fusarium oxysporum) and Fat15 (Alternaria sp.) were screened to be the most effective candidates for promoting F. tataricum sprout growth and rutin production. With application of polysaccharide (PS, 150 mg/l) of endophyte Fat6, PS (200 mg/l) of endophyte Fat9, and PS (150 mg/l) of endophyte Fat15, the rutin yield was effectively increased to 47.89 mg/(100 sprouts), 45.85 mg/(100 sprouts) and 46.83 mg/(100 sprouts), respectively. That was about 1.5- to 1.6-fold compared to the control culture of 29.37 mg/(100 sprouts). Furthermore, the present study revealed that the biosynthesis of the functional flavonoid resulted from the stimulation of the phenylpropanoid pathway by mycelia polysaccharide treatments. Application of specific fungal elicitors could be an efficient strategy for improving the nutritional and functional quality of tartary buckwheat sprouts.