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Ficker, L. A., Kirkness, C. M., Rice, N. S., et al.: Longterm prognosis for corneal grafting in herpes simplex keratitis. Eye (Lond.), 1988, 2 , 400

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Orvosi Hetilap
Authors: Ilona Mihály, Tímea Kolozsi, Zoltán Liptai, Adrienn Lukács, Péter Molnár, József Budai, Géza Prinz, Anita Ábrahám, Miklósné Palánszky and Józsefné Dóczy

Whitley, R. J., Kimberlin, D. W., Roizman, B.: Herpes simplex viruses. Clin. Infect. Dis., 1998, 26 , 541–555. Roizman B

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To compare the immunogenity of the herpes simplex virus 1 (HSV-1/HHV-1) recombinant glycoprotein D (gD1), as a potential protective vaccine, Balb/c mice were immunized with either gD1/313 (the ectodomain of the gD1 fusion protein consisting of 313 amino acid residues), or the plasmid pcDNA3.1-gD (coding for a full length gD1 protein, FLgD1). A live attenuated HSV-l (deleted in the gE gene), and a HSV-1 (strain HSZP)-infected cell extract served as positive controls, and three non-structural recombinant HSV-1 fusion proteins (ICP27, UL9/OBP and thymidine kinase - TK) were used as presumed non-protective (negative) controls. Protection tests showed that the LD 50 value of the challenging infectious virus increased 90-fold in mice immunized with ICP27, but remained unchanged in other control mice immunized with TK and OBP polypeptides. A significant protection (the LD 50 value of challenging virus increased 800-fold) was noted following immunization with gD1/313, while immunization with the gE-del virus and/or the gD1 DNA vaccine resulted in a more than 4,000-fold increase of the challenging virus dose killing 50% of the animals. Using ELISA, elevated antibody titers were detected following immunizations with gD1/313, gE-del virus, and/or HSV-1-infected-cell extract. In addition, all of the three non-structural proteins elicited a good humoral response (with titres ranging from 1:16,000 to 1:128,000). The lowest IgG response (1:8,000) was noted after immunization with the gD1 DNA vaccine. Peripheral blood leukocytes (PBLs) as well as splenocytes from mice immunized with gD1/313, gE-del virus, and gD1-plasmid responded in lymphocyte transformation test (LTT) to the presence of purified gD1/313 antigen. For PBLs, the most significant stimulation of thymidine incorporation was registered at a gD1/313 concentration of 5 µg/100 µl, while the splenocytes from DNA vaccine-immunized mice responded already at a concentration of 1 µg/100 µl.

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Whitley, R. J.: Herpes simplex viruses In: Knipe, D. M., Howley, P. M. (eds): Fields’ Virology, Lippincott Williams & Wilkins, Philadelphia, pp. 2461–2510 (2001). Whitley

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. Buchmann , K. and Mehrdana , F. ( 2016 ): Effects of anisakid nematodes Anisakis simplex (s.l.), Pseudoterranova decipiens (s.l.) and Contracaecum osculatum (s.l.) on fish and consumer health . FAWPAR 4 , 13 – 12

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–75. 28 Kaewpoowat Q, Salazar L, Aguilera E, et al. Herpes simplex and varicella zoster CNS infections: clinical presentations, treatments and outcomes. Infection 2016; 44: 337

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Virology, 3rd ed., Lipincott, Raven, 1996, pp. 173-217. Fields Virology 173 217 Whitley, R. J.: Herpes simplex

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Koelle, D.M., Corey, L.: Herpes simplex: Insights on pathogenesis and possible vaccines. Annu Rev Med 59 , 381–395 (2008). Corey L. Herpes simplex: Insights on pathogenesis and

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2291 2296 Aubert, M. and Blaho, A. J. (2001): Modulation of apoptosis during herpes simplex virus infection in human cells. Microbes Infect. 3 , 859

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water for 10 min. Lastly, centrifuge step was operated at 12,000 rpm at 4 °C for 10 min. The supernatant containing DNA was transferred to a sterile micro tube and stored at −20 °C. All isolates were screened by simplex PCR for the following β

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