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105 Vajta, G., Booth, P. J., Holm, P., Greve, T. and Callesen, H. (1997): Successful vitrification of early stage bovine in vitro produced embryos with the open-pulled straw (OPS) method
Bali Papp, Á., Somfai, P., Varga, E. and Marosán, M. (2005): Survival of porcine oocytes at germinal vesicle stage after vitrification with open pulled straw method. Reprod. Fert. Dev. 17
291 297 Chen, S., Ge, W., Buswell, G. A.: Molecular cloning of a new laccase from the edible straw mushroom Volvariella volvacea: possible involvement in fruit body
Vajta, G., Holm, P., Kuwayama, M., Booth, P. J., Jacobsen, H., Greve, T. and Callesen, H. (1998): Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev. 51 , 53
90 547 553 Chen, S., Lien, Y., Chen, H., Chao, K., Ho, H. and Yang, Y. (2000): Open pulled straws for vitrification of mouse mature oocytes
Lopez-Bejar, M. and Lopez-Gatius, F. (2002): Nonequilibrium cryopreservation of rabbit embryos using a modified (sealed) open pulled straw procedure. Theriogenology 58 , 1541–1552. Lopez-Gatius F
192 Berthelot, F., Martinat-Botte, F., Locatelli, A., Perreau, C. and Terqui, M. (2000): Piglets born after vitrification of embryos using the open pulled straw method. Cryobiology 41
This paper describes the production of an enzyme preparation from the fungus Thermomyces lanuginosus. Thermal resistance, pH stability and lignocellulolytic activity of the enzyme preparation high in xylanase were studied on a variety of grains and forages. The enzyme preparation preserved more than 70% of its original xylanase activity for 4 and 1 h at 60 and 70 °C, respectively. The xylanase activity remained over 80% when the preparation was incubated for 30 min at pH 4.5. In vitro digestibility studies indicated that the enzyme digested 7.5, 8.5 and 8.0% of the dry matter (DM) of barley meal, wheat bran and oat meal samples, respectively. When applying 60-min incubation, 7.5, 7.3 and 8.4% of DM of the oat straw, alfalfa hay and triticale straw was digested, respectively. When the time of digestion was increased to 360 min, the sunflower hull showed 15.8% DM digestibility.
://commons.wikimedia.org/wiki/File%3AVanGogh-starry_night_ballance1.jpg Even more interesting is his yellow period [ Vase with Fifteen Sunflowers (1888) or self-portraits with straw hat]. In these pictures, the environment and the persons received
The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 ± 8.21%), CASA motility (12.2 ± 5.69%) and progressive motility (3.52 ± 2.13%), compared with the controls (43 ± 2.73%, 55.4 ± 6.78% and 33.48 ± 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 ± 3.99% and 44.1 ± 2.18%) compared with the control (13 ± 8.15 and 31.7 ± 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.
