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A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 μg/g for cotinine, 20 μg/g for anabasine and nornicotine, and 30 μg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

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. A new solution for an old problem: the regiochemical distribution of the acyl chains in galactolipids can be established by electrospray ionization tandem mass spectrometry Rapid Commun . Mass Spectrom. 17 : 1982 – 1994

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Acta Chromatographica
Authors: Lianguo Chen, Qingwei Zhang, Yijing Lin, Xiaojie Lu, Zuoquan Zhong, Jianshe Ma, Congcong Wen, and Cheng Ding

necessary to establish an analytical method to monitor the concentration of hapepunine. To the best of our knowledge, the pharmacokinetics of hapepunine had not been reported. In this paper, an ultra-performance liquid chromatography–tandem mass

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C HEN , G. , C AO , P. & LIU , R. ( 2011 ): A multi-residue method for fast determination of pesticides in tea by ultraperformance liquid chromatography-electrospray tandem mass spectrometry combined with modified QuEChERS sample preparation

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spectrometry (MS) [ 18 , 19 ], or coupled to tandem mass spectrometry (MS/MS) [ 6 , 18 , 20 – 23 ], had become the best choice for the determination of CAP because of its advantages of high selectivity and sensitivity, timesaving, and the accuracy of

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/sample. The injection volume was 10 μL. Electrospray ionization–tandem mass spectrometry (ESI–MS/MS) detection was achieved on an Agilent 6410 triple quadrupole tandem mass spectrometer. The positive ionization mode was used, and the ions were

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. Therefore, we attempted to develop and validate a sensitive and specific analytical method for the simultaneous determination of ZnPT and PT in shampoos using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Experimental

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Acta Chromatographica
Authors: Ruijie Chen, Mengrou Lu, Xiaoting Tu, Wei Sun, Weijian Ye, Jianshe Ma, Congcong Wen, Xianqin Wang, and Peiwu Geng

]. There is no effective bioanalytical method available to quantify panasenoside in biological samples to date. Compared to liquid chromatography tandem mass spectrometry (LC–MS/MS), ultra-performance liquid chromatography (UPLC)–MS/MS is more sensitive and

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]. LC coupled to an ultraviolet (UV) source or diode array [ 16 , 18 , 19 ], fluorescence [ 20 ], a time-of-flight (TOF) analyzer [ 21 ], or biosensor [ 22 ] has been used to detect antibiotics. Meanwhile, LC coupled to tandem mass spectrometry (LC

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humans, they both used [ 14 C]-labeled apremilast rather than normal tablets [ 15 ]. Liu’s method has been used to study the drug interactions with apremilast by liquid chromatography-tandem mass spectrometry [ 16, 17 ]. However, no study has reported

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