Authors:Z. Šťástková, R. Karpíšková, T. Gelbíčová, V. Vaňáč, Š. Tůma and B. Světlíková
Ruzickova, V., Karpiskova, R., Pantucek, R., Pospisilova, M., Cernikova, P. & Doskar, J. (2008): Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in theCzechRepublic. Int. J. Fd Microbiol. , 121 , 60
Authors:J. Cetkovská, P. Diviš, M. Vespalcová, J. Pořízka and V. Řezníček
The ascorbic acid, total polyphenols, total anthocyanins and mineral content, together with antioxidant activity, was determined in five Czech, two Ukrainian and two Austrian cultivars of cornelian cherry (Cornus mas L.) widely grown in the Czech Republic. Ascorbic acid content varied between 199–433 mg kg−1, total polyphenols between 2174–6143 mg kg−1, and total anthocyanins between 61–253 mg kg−1. All fruits were good sources of major metals (K, Ca, Mg, Fe, and Mn) and trace elements (Cu, Zn, and Cr). The antioxidant activity was determined by EPR and DPPH radical scavenging assay and ranged from 29.5% to 67.2%. There was a linear relationship between antioxidant activity and total polyphenol content. Based on the obtained results, Ekotišnovský, Fruchtal, and Ruzyňský cultivars were recommended for further investigation and breeding programme of cornelian cherry fruit in the Czech Republic.
Authors:J. Varga, Z. Koncz, S. Kocsubé, T. Mátrai, J. Téren, V. Ostry, J. Skarkova, J. Ruprich, A. Kubatova and Z. Kozakiewicz
Ostry, V., Skarkova, J., Malir, F. & Sycorova, S.
(2004): Advances on the occurrence of toxigenic fungi and mycotoxins in theCzechRepublic.-in: Logrieco, A. & Visconti, A.
An overview on toxigenic fungi and mycotoxins in Europe
Authors:Marie Rusinová, Miloš Kalousek and Miloš Lavický
Wooden structures are the oldest building elements accompanying man since time immemorial. The Czech Republic is strict in its legal and normative regulations relating to the use of wooden building components. In spite of that the Czech construction market is increasingly returning to wooden houses; not only for dwelling purposes, but also for public amenities. However, the structures made of wood as combustible material have to be carefully assessed especially from the viewpoint of fire safety of the building. This paper deals with the fire safety of wooden structures with special regard to the suspended type of ceiling, as it fulfils the role of insulation against the effects of fire. In particular, the thermal stress caused by fire and its impact upon the safety of the building elements has been investigated.
Determination of consumers’ acceptance level of sushi meal among Czech respondents was the main aim. The survey included 1352 respondents that filled in a questionnaire on their demographic characteristics and food preferences regarding their acceptance of sushi meal. Additionally, 79 volunteers participated in sorting sushi among other 14 popular meals in the Czech Republic, according to their assumed situations. The results indicate that sushi is highly accepted among Czech consumers (more than 80% of respondents consume sushi) due to sensory characteristics and health benefit claims of sushi. The main barrier for sushi acceptance is sushi being a cold meal. The study emphasized that sushi price highly influences not only consumption frequency but also acceptance of sushi among respondents who declared not consuming this type of meal.
The important glycoalkaloids in potatoes are α-solanine and α-chaconine. Their natural function is probably to serve as stress metabolites or phytoalexins for the protection of the potato when attacked by insects, fungi, etc. They contribute flavour to potatoes but at higher concentrations cause bitterness and are toxic to humans. α-Solanine and α-chaconine appear to have two main toxic actions, one on cell membranes and another one on acetylcholinesterase. Symptoms of α-solanine/α-chaconine poisoning involve an acute gastrointestinal upset with diarrhea, vomiting and severe abdominal pain. An instrumental high-performance thin-layer chromatography (HPTLC) method was applied for the quantification of α-solanine and α-chaconine in peeled potato skin, raw potato pulp and cooked peeled potato tubers. The limit of quantification (LOQ) for α-solanine and α-chaconine was found to be 5.0 mg kg
for each glycoalkaloid. In this study the factors of potential loss of α-solanine and α-chaconine in potato tubers during peeling (factor=0.8) and cooking into edible stage in salted water (factor=0.8) were examined. The combined loss factor of peeling and cooking for sum of both glycoalkaloids in potato tubers was 0.64. These factors were practically used for the probabilistic exposure assessment of the intake of potato glycoalkaloids in the Czech Republic.
Authors:V. Kyrova, V. Ostry, L. Laichmannova and J. Ruprich
Genetically modified (GM) plants are obtained by adding to them one or more foreign genes that encode new properties, such as tolerance to herbicides, resistance to insects and the ability to produce new substances. The aim of this study was the detection and identification of GM foodstuffs. Six different types of samples (soybeans, soya products, tomatoes, maize flour, rice and papaya) were collected at 12 places in the Czech Republic during the years 2002–2007. It represents a total of 1225 samples of foodstuffs.Samples were investigated for the presence of material derived from the following genetically modified organisms (GMOs) which are approved for food use in the European Union (EU): Roundup Ready soybean (RRS) and maize lines Bt176, Bt11, T25, GA21, MON810, DAS1507 and some non-approved in the EU: maize lines Bt10 and starlink, rice, tomatoes and papaya. Polymerase chain reaction (PCR)-based methods and enzyme-linked immunosorbent assay (ELISA) were used for the detection of GM foods.RRS was detected in 14 (4.9%) samples of soybean out of 288 and in 88 (30.5%) soya products out of 288 samples. The amount of RRS in positive samples was determined by quantitative PCR. The content was in the range of 0.01–75.3% RRS. GM maize was detected in 5 (1.7%) of 288 samples. Maize lines MON810, Bt176 and StarLink were detected in the maize samples. GM rice was detected in 2 (1.9%) samples out of 102. All investigated tomatoes and papaya samples were negative for detection of GM.