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.H. & Yesil-Celiktas , O. ( 2009 ): Supercritical CO 2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization . J. Supercrit. Fluid. , 51 , 29 – 35

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Authors: P. A. S. Theophilus, M. J. Victoria, K. M. Socarras, K. R. Filush, K. Gupta, D. F. Luecke and E. Sapi

. Ghosh S , Subudhi E , Nayak S : Antimicrobial assay of Stevia rebaudiana Bertoni leaf extracts against 10 pathogens plant materials and microorganisms determination of minimum inhibitory concentration (MIC) . Int J

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Summary

In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL·min−1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1H NMR, and 13C NMR.

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Mahlberg, P. G. (2001): Development and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert.-Asteraceae. — Revista Brasil. Bot. 24 (3): 349–357. Mahlberg P. G

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Stevia rebaudiana Bertoni on oxidative stress in type-2 diabetic rat models. Biomed. Aging Pathol., 2012, 2 (3), 126–131. 28 Shivanna, N., Naika, M., Khanum, F., et

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ingredients. Off. J. Eur. Comm., 1997, L43, 1–6. 71 Commission Decision of 22 February 2000 refusing the placing on the market of Stevia rebaudiana Bertoni plants and dried leaves as

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Authors: Eva Sapi, Priyanka A. S. Theophilus, Truc V. Pham, Divya Burugu and David F. Luecke

, Victoria MJ , Socarras KM , Filush KR , Gupta K , Luecke DF , Sapi E : Effectiveness of Stevia rebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro . Eur J of Microbiol and Immunol 5 , 268 – 280

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Authors: E. Sapi, K. Gupta, K. Wawrzeniak, G. Gaur, J. Torres, K. Filush, A. Melillo and B. Zelger

-6736(01)05321-1 80. Theophilus PA , Victoria MJ , Socarras KM , Filsuh KR , Gupta K , Luecke DF : Effectiveness of stevia rebaudiana whole leaf extract against the various

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