Authors:P. A. S. Theophilus, M. J. Victoria, K. M. Socarras, K. R. Filush, K. Gupta, D. F. Luecke and E. Sapi
, Subudhi E , Nayak S : Antimicrobial assay of Steviarebaudiana Bertoni leaf extracts against 10 pathogens plant materials and microorganisms determination of minimum inhibitory concentration (MIC) . Int J
Authors:X. H. Lv, P. Q. Kuang, Q. P. Yuan, H. Liang and G. Q. Zheng
In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL·min−1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1H NMR, and 13C NMR.
Authors:Eva Sapi, Priyanka A. S. Theophilus, Truc V. Pham, Divya Burugu and David F. Luecke
, Victoria MJ , Socarras KM , Filush KR , Gupta K , Luecke DF , Sapi E : Effectiveness of Steviarebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro . Eur J of Microbiol and Immunol 5 , 268 – 280