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Tannase an industrially important enzyme was produced by Aspergillus aculeatus DBF9 through a solid-state fermentation (SSF). The organism produced good amount of enzyme and gallic acid in wheat bran among the solid substrate used in SSF. Maximum enzyme and gallic acid production occurred in 5% tannic acid after 72 h. Eighty percent initial substrate moisture and 30 °C temperature was found suitable for tannase production.

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., Fernandez-Arrojo, L., Garcia-Arellano, H., Ferrer, M., Ballestoros, A. & Plou, F.J. (2007): Purification and kinetic characterization of fructosyltransferase from Aspergillus aculeatus. J. Biotechnol., 128, 204

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. Materials and methods Materials Food-grade sucrose manufactured by Pfeifer and Lanngen KG (Köln, Germany) was purchased from a local food store. Pectinex Ultra SP-L, a commercial enzyme preparation from Aspergillus aculeatus , was supplied by Novozyme A

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Hamari, Zs., Kevei, F., Kovács, É., Varga, J., Kozakiewicz, Z., Croft, J. H.: Molecular and phenotypic characterisation of Aspergillus japonicus and Aspergillus aculeatus strains with special regard to their mitochondrial DNA polymorphisms. Antonie

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. & S u , W. ( 2013 ): Purification and characterization of a naringinase from Aspergillus aculeatus JMUdb058 . J. Agr. Food Chem. , 61 , 931 – 938 . H avas , P. , K un , S z

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) and pectinase from Aspergillus aculeatus with activity >3800 U ml –1 (Pectinex Ultra SPL, Novozymes) were used. 1.2 Extraction Different techniques of anthocyanins extraction (maceration, ultrasound-assisted extraction UAE, enzyme

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