therapy. Then, if there is no need to pay attention to its safety while taking CUR or if there is any clause that we have to follow when it comes to drug combination?
Bacillussubtilis ( B. subtilis ), which is commonly found in soil and
Authors:J. Farkas, É. Andrássy, A. Simon, and L. Mészáros
retardation and thermal disinfection of Bacillussubtilis spores by hydrostatic pressure. J. Bacteriol , 57 , 353-358.
The retardation and thermal disinfection of Bacillussubtilis spores by hydrostatic pressure
Authors:Sándor Nagy, Béla Kocsis, Tamás Kőszegi, and Lajos Botz
The main purpose of this study was to determine optimum conditions for culture of a test microbe
(ATCC 6633) which enabled us to establish its use for direct bioautography. The viability of the bacteria on TLC plates was measured on the basis of their adenosine-5′-triphosphate (ATP) content as determined by bioluminescent luciferin/luciferase assay, the data being referred to values for total bacterial protein. In the first experiments, we used a ‘20-h’ culture of
prepared by dilution of an optical density (
) ≫ 0.4 culture to furnish a culture of
= 0.4 (Method A). Later, on the basis of our optimization experiments we found that a ‘5–9-h’ broth culture of
was suitable. Under these conditions the bacteria remained in the log phase (
= 0.2–0.4) for 5–9 h (Method B) in immersion bacterial suspension. Because the test bacteria were in the log phase a much shorter incubation time (4–8 h) was sufficient for TLC plates instead of the original 18 h in a previous study. One advantage of this method, in addition to the shorter incubation time, is that we can use TLC plates coated with adsorbents other than silica.