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. Ghosh S , Subudhi E , Nayak S : Antimicrobial assay of Stevia rebaudiana Bertoni leaf extracts against 10 pathogens plant materials and microorganisms determination of minimum inhibitory concentration (MIC) . Int J

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In existing processes, the extraction of steviol glycosides from stevia leaves involves many process steps often including extraction by organic solvents. The purpose of the present study was to develop a process for the effective extraction of steviol glycosides, which can provide a concentrated juice exhibiting a high level of recovery with regard to the target compounds, rebaudioside A and stevioside. Pressurized Hot Water Extraction (PHWE) was first optimized with Response Surface Methodology in terms of maximized rebaudioside-A yield and minimized colour components. PHWE was then combined with pressing in a wine-press, resulting higher efficiency for extracting both steviol glycosides in comparison to the reported methods in the literature. Finally, spray drying was applied for both product stabilization and removal of contaminants.

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Summary

In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL·min−1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1H NMR, and 13C NMR.

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Stevia rebaudiana Bertoni on oxidative stress in type-2 diabetic rat models. Biomed. Aging Pathol., 2012, 2 (3), 126–131. 28 Shivanna, N., Naika, M., Khanum, F., et

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ingredients. Off. J. Eur. Comm., 1997, L43, 1–6. 71 Commission Decision of 22 February 2000 refusing the placing on the market of Stevia rebaudiana Bertoni plants and dried leaves as

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, Victoria MJ , Socarras KM , Filush KR , Gupta K , Luecke DF , Sapi E : Effectiveness of Stevia rebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro . Eur J of Microbiol and Immunol 5 , 268 – 280

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. Rebaudioside-A is extracted from leaves of Stevia rebaudiana and has a relative sweetness 250–300 times higher than sucrose ( Gao et al., 2017 ). Erythritol is a four carbon sugar alcohol, stable at baking temperature, but has to be used with a bulking agent

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European Journal of Microbiology and Immunology
Authors:
E. Sapi
,
K. Gupta
,
K. Wawrzeniak
,
G. Gaur
,
J. Torres
,
K. Filush
,
A. Melillo
, and
B. Zelger

, Filsuh KR , Gupta K , Luecke DF : Effectiveness of stevia rebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro . Eur J Microbiol Immunol (Bp) . 2015 ; 5 : 268 – 80 . 10

Open access