, Subudhi E , Nayak S : Antimicrobial assay of Steviarebaudiana Bertoni leaf extracts against 10 pathogens plant materials and microorganisms determination of minimum inhibitory concentration (MIC) . Int J
In existing processes, the extraction of steviol glycosides from stevia leaves involves many process steps often including extraction by organic solvents. The purpose of the present study was to develop a process for the effective extraction of steviol glycosides, which can provide a concentrated juice exhibiting a high level of recovery with regard to the target compounds, rebaudioside A and stevioside. Pressurized Hot Water Extraction (PHWE) was first optimized with Response Surface Methodology in terms of maximized rebaudioside-A yield and minimized colour components. PHWE was then combined with pressing in a wine-press, resulting higher efficiency for extracting both steviol glycosides in comparison to the reported methods in the literature. Finally, spray drying was applied for both product stabilization and removal of contaminants.
In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL·min−1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1H NMR, and 13C NMR.
, Victoria MJ , Socarras KM , Filush KR , Gupta K , Luecke DF , Sapi E : Effectiveness of Steviarebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro . Eur J of Microbiol and Immunol 5 , 268 – 280
. Rebaudioside-A is extracted from leaves of Steviarebaudiana and has a relative sweetness 250–300 times higher than sucrose ( Gao et al., 2017 ). Erythritol is a four carbon sugar alcohol, stable at baking temperature, but has to be used with a bulking agent