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It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells.

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5 mediated reduction. Meat Sci. , 50 , 333–342. Buckley D.J. A potential mechanism by which α-tocopherol maintains oxymyoglobin pigment through cytochrome b5 mediated

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Illékony nitritszármazékok („popperek”) által okozott methaemoglobinaemia

Methemoglobinemia caused by volatile nitrites derivates (‘poppers’)

Orvosi Hetilap
Authors: Ágnes Bakos and Anna Bátyi

methaemoglobinaemia: cytochrome b5 reductase deficiency. Br J Haematol. 2008; 141: 298–308. 19 Yamaji F, Soeda A, Shibata H, et al. A new mutation of congenital

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Acta Biologica Hungarica
Authors: Nikoletta Szabó, Jovana Ajduković, Evgenija Djurendić, Marija Sakač, Imre Ignáth, János Gardi, Gábor Mahmoud, Olivera Klisurić, Suzana Jovanović-Šanta, Katarina Penov Gaši, and Mihály Szécsi

. 2008 26 4563 4571 Auchus, R. J., Lee, T. C., Miller, W. L. (1998) Cytochrome b5 augments the 17,20-lyase activity of

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