Authors:K. Szentmihályi, M. Szilágyi, J. Balla, L. Ujhelyi, and A. Blázovics
Kögl , C., Schneider , W. & Elstner , E.F. (1994): The influence of magnesium-pyridoxal-5’-phosphate-glutamate in comparison with probucol, α-tocopherol and trolox on copper-induced oxidation of human low density lipoprotein invitro . Biochem
Authors:Clara Malo, Lydia Gil, Rafael Cano, Felisa Martinez, and Noelia Gonzalez
Abeydeera, L. R., Wang, W. H., Prather, R. S. and Day, B. N. (1998): Maturation invitro of pig oocytes in protein-free culture media: Fertilization and subsequent embryo development invitro . Biol. Reprod
Authors:P. Sharma, H.K. Chaudhary, N.V. Manoj, and P. Kumar
An investigation to standardize the protocol for in vitro application of colchicine for enhancing the doubled haploid production in wheat was done. Two tetraploid (PDW-314 and A-9-30-1); and two hexaploid (DH-40 and C-306) wheat genotypes were used as maternal parents, whereas, the pollen sources involved Zea mays (cv. Bajaura Makka) and Imperata cylindrica. During the rabi seasons of years 2013–14 and 2014–15, wheat × maize and wheat × I. cylindrica hybridization was carried out followed by treatment of their haploids produced as a result of elimination of chromosomes of maize and I. cylindrica respectively, with varied doses of colchicine for different durations The various doses of colchicine were categorized into two groups: lower doses for longer durations (0.01, 0.025, 0.05% each for 5, 7, 9, 11 hrs) and higher doses for shorter durations (0.05, 0.075, 0.10, 0.15, 0.20, 0.25% each for 5, 4, 3, 2 hrs). The response of different concentrations of colchicine applied for varied durations revealed significant differences for various doubled haploidy parameters viz., per cent survived plants, per cent doubled haploid formation and per cent doubled haploid seed formation. In hexaploid and tetraploid wheats, colchicine doses of 0.075% for 4 hrs and 0.15% for 4 hrs, respectively were established as optimum for enhanced doubled haploid production.