Authors:Venkata Ramana Malipeddi, Rajendra Awasthi, and Kamal Dua
is using polymeric microspheres as drug carriers [1, 2] .
Metoprolol tartrate is a selective β-adrenergic receptor blocking agent and commonly used for the treatment of mild to moderate hypertension and stable angina. It is beneficial in
Authors:Alireza Kamali, Amir Sanatkar, Mehrzad Sharifi, and Esmaeil Moshir
study the effectiveness of metoprolol and amiodarone (which are commonly used to prevent POAF) in treating POAF.
Materials and Methods
This is a double-blind, randomized, clinical trial conducted on the patients who were
products for which no Minilab manual or FDA Compendium methods have been published: the anti-arrhythmic agent amiodarone HCl (CAS No. 19774-82-4); the β blockers carvedilol (CAS No. 72956-09-3), metoprolol succinate (CAS No. 98418-47-4), and nebivolol HCl
Authors:Branka Lučić, Dušanka Radulović, Zorica Vujić, and Danica Agbaba
A method for chromatographic separation of the enantiomers of (±)-metoprolol tartrate (±MeT) employing a chiral mobile phase additive (CMPA) is described. By using silica gel plates previously impregnated with the mobile phase (ethanol-water, 70 + 30,
) containing D
-(−)-tartaric acid as chiral selector, direct separation of the enantiomers of ±MeT was achieved. The results of experiments with different concentrations of D
-(−)-tartaric acid (5.8, 11.6, and 23 mmol L
) revealed that the best resolution of the enantiomers of ±MeT was achieved with 11.6 mm D
-(−)-tartaric acid in both the mobile phase and the impregnation solution, at 25 ± 2°C.Spot visualization on chromatograms was performed by use of a fixed-wavelength (
= 254 nm) ultraviolet lamp or an iodine vapor-saturated chamber.
A simple, rapid, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method for simultaneous analysis of metoprolol succinate (MET) and amlodipine besylate (AMLO) in pharmaceutical preparations has been established and validated. Separation was achieved on silica gel 60 F
plates with methanolethyl acetate-water-toluene-25% ammonia 1.5:5.0:0.3:3.0:0.3 (
) as mobile phase. Densitometric quantification was performed at 236 nm by reflectance scanning. The
values of MET and AMLO were 0.31 and 0.43, respectively. The linearity of the method was investigated in the range 180 to 280 μg mL
for MET and 18 to 28 μg mL
for AMLO. Recovery for MET and AMLO was, respectively, 99.76% and 99.15% by peak height and 99.72% and 99.22% by peak area. The method was validated for precision, accuracy, specificity, and ruggedness.
A simple and rapid method has been established for indirect separation of the enantiomers of (R,S)-metoprolol and (R,S)-carvedilol by reversed-phase TLC. Beta blockers derivatized with 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (Marfey’s reagent, FDNP-l-Ala-NH2) and its six structural variants (FDNP-l-Phe-NH2, FDNP-l-Val-NH2, FDNP-l-Pro-NH2, FDNP-l-Leu-NH2, FDNP-l-Met-NH2, and FDNP-d-Phg-NH2) were spotted on precoated plates. (R,S)- Metoprolol and (R,S)-carvedilol were isolated from pharmaceutical dosage forms and purified. The diastereomers were separated most effectively by use of mobile phases containing acetonitrile and triethylamine-phosphate buffer (50 mM, pH 5.5). The results obtained by use of Marfey’s reagent were compared with those obtained by use of the other variants. The effects of buffer concentration, pH, and concentration of organic modifier were studied.
Resolution of racemic metoprolol, propranolol, carvedilol, bisoprolol, salbutamol, and labetalol, commonly used β-blockers, into their enantiomers has been achieved by TLC on silica gel plates impregnated with optically pure L
-Glu and L
-Asp. Acetonitrile-methanol-water-dichloromethane and acetonitrile-methanol-water-glacial acetic acid mobile phases in different proportions enabled successful separation. The spots were detected with iodine vapor. The detection limits were 0.23, 0.1, 0.27, 0.25, 0.2, and 0.2 μg for each enantiomer of metoprolol, propranolol, carvedilol, bisoprolol, salbutamol, and labetalol, respectively.