β-Glucan content and β-glucanase activity of winter and spring barley cultivars grown under different environments were evaluated. There were significant differences in both β-glucan content and β-glucanase activity between analysed barleys. The results showed that, for all cultivars and locations, approximately 75% of β-glucan present in grains was degraded after malting, and that marked differences existed among winter and spring type of cultivars in malt β-glucan content. The correlation analysis of β-glucan content and malt quality parameters showed that malt β-glucan content was significantly positively correlated with viscosity and extract difference, and negatively with malt β-glucanase activity and friability. Regarding malt β-glucanase activity, significantly higher activity was found in spring cultivars in contrast to winter cultivars.