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Abstract  

Differential scanning calorimetry (DSC) has been employed to study the thermal denaturation processes of the main protein fractions of blood serum. These processes have been compared for albumins (nondefatted (HSA) and fatty acid free (HSAf)), α,β-globulins, γ-globulins, and their mixtures in aqueous (pH 6.5) and buffer (pH 7.2) solutions. The results have indicated that α,β-globulins inhibit γ-globulins’ aggregation in buffer solutions. The decrease of stability of HSA and HSAf aqueous solutions has been observed in the presence of γ-globulins. The mixtures of albumins and γ-globulins have revealed the tendency to ready aggregation in water. Moreover, the results have suggested that neither γ-globulins nor albumins severely change the stability of α,β-globulins.

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Acta Veterinaria Hungarica
Authors: Marek Opalka, Justyna Kugla-Owczarska, Barbara Kaminska, Helena Puchajda-Skowronska, Wioletta Hryniewicka, and Luiza Dusza

Szafranska, B., Ziecik, A. and Okrasa, S. (2002): Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins — an available tool for studies of reproductive processes. Reprod. Biol. 2 , 187

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Szafranska, B., Ziecik, A. J. and Okrasa, S. (2002): Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins — an available tool for studies of reproductive processes. Reprod. Biol. 5 , 187

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): Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins — an available tool for studies of reproductive processes. Reprod. Biol. 2 , 187–203. Okrasa S

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719 Szafrańska, B., Zięcik, A. J. and Okrasa, S. (2002): Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins-an available tool for studies of

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244 Szafrańska, B., Zięcik, A. and Okrasa, S. (2002): Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins — an available tool for studies of

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. Szafranska , B. , Ziecik , A. and Okrasa , S. ( 2001 ): Polyclonal primary antisera against various steroids (E17P, Eb A4, T, B, F) or proteins (pLH, pPAG) and polyvalent secondary antisera against γ-globulins - as available suitable tools required

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γ-globulins -- an available tool for studies of reproductive processes. Reprod. Biol. 2 , 97--113. Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins -- an available tool for

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