Six different 1RS-specific molecular markers (RMS13, Bmac213, GPI, 5S, SCM9, IAG95) were tested in twenty rye cultivars of various origin. The aim of the experiments was to choose rye cultivars which give polymorphic PCR products with these 1RS-specific markers compared to the wheat cultivar Mv Magdaléna, which contains the 1BL.1RS translocation. The polymorphic rye cultivars can be presumed to differ from the 1BL.1RS translocation originating from the Petkus rye cultivar and will hopefully carry effective resistance genes which can be incorporated into the 1BL.1RS translocation in wheat. Twenty rye cultivars (at least two plants per cultivar) were analysed with these markers. Of fifty-two rye samples analysed, three plants were found to be polymorphic, one (Kisvárdai Alacsony from Hungary), for the 5S marker, one (Kriszta from Hungary) for the RMS13 marker and one (Porto from Portugal) for the SCM9 marker. The polymorphic plants were grown to maturity in the phytotron.