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The nucleotide sequence of the coat protein (CP) gene and the 3' non-translated region, in relation to aphid transmission of 7 potato tuber necrotic ringspot isolates of Potato virus Y (PVYNTN) were studied. Five isolates originated from different areas of potato fields in Hungary and two German isolates served as controls. A 5' tail of the nucleotide sequences of the CP region and 3' non-translated region (NTR) were determined. Sequence data were sent to the EMBL GeneBank Database. Homology of nucleotide and amino acid sequences were high among the studied PVY isolates. According to the characteristic regions, all isolates belonged to the PVYNTN strain. All of the tested isolates could be transmitted by the aphid Myzus persicae Sulzer to the test plant Nicotiana tabacum L. verifying the wide distribution of tuber necrotic ringspot strain in Hungary. Our data suggest that the high homology found in the CP region of the different isolates, are suitable for development of coat protein mediated resistance against PVY in commercially important host plants like, e.g. potato.

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Acta Microbiologica et Immunologica Hungarica
Authors: Enikő Fehér, Szilvia Marton, Ádám György Tóth, Krisztina Ursu, Kerstin Wernike, Martin Beer, Ádám Dán, and Krisztián Bányai

sequence (SBV-Hun1, SBV-Hun3, SBV-Hun5, and SBV-Hun6), whereas the other three were single nt mutations of variable sequences. An additional nt change (G755A) was detected in the 3non-coding region (NCR) of the strain SBV-Hun1. The nt and aa identities

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