In industrial production of button mushroom
, the green mold epidemics by
species cause serious damage. Sampling has preformed in various stages from pili, compost, seed and soil.
selective media and PDA were used to isolate
species. Out of 423 isolates, three sections of
Trichoderma (Trichoderma, Pachybasium
were identified.Among these isolates, more than 350 related to
section, 35 to
section and 20 related to
T. harzianum, T. virens, T. atroviride, T. citrinoviride, T. ghanens
were identified from
. No isolates of
were obtained in this study.
. The high-resolution fingerprinting method of amplified fragment length polymorphism (AFLP) analysis and sequencing of ITS regions were used to study the genetic relationships among
isolates. A total of 1127 polymorphic AFLP loci were obtained using 20 primer combinations. No clear trend was detected between clustering in AFLP dendrogram and geographic origin of isolated materials. According to AFLP and morphological analysis, it is concluded that application of these markers resulted the same clustering and also genetic diversity in
aggregate group proved byAFLP, suggesting thatAFLP is suitable marker along with sequencing of conserved regions for complementary classification of
with other phylogenetic characteristics.
Medini, M., Hamza, S., Rebai, A., Baum, M. 2005. Analysis of genetic diversity in Tunisian durum wheat cultivars and related wild species by SSR and AFLPmarkers. Genet. Res. Crop Evol.
Authors:S. Marghali, H. Chennaoui, H. Bourguiba, M. Marrakchi, and N. Trifi-Farah
Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding molecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP fragments was followed in F2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit×El Haouaria, which had previously characterised by morphological and AFLP markers. A comparison of profiles from the morphologically contrasted parental individuals and F2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver-stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum forage crop.
Authors:G. Surányi, C. Máthé, Ágnes Mosolygó, G. Borbély, and G. Vasas
Crocus taxonomy has until now been based primarily on morphology, taking chromosome numbers into consideration. The genetics and genome structure of the genus, the relationships and diversity within the genus are not well known. Amplified fragment length polymorphism (AFLP) is a whole genome approach to study genetic variation that is gaining in popularity for lower-level systematics. The present study employed the AFLP technique for analyzing relationships among taxa of the Crocus genus (particularly the Crocus vernus aggregate) with Carpathian Basin origin. The molecular variance obtained was based on amplification, separation and detection of EcoRI and Tru1I double-digested Crocus spp. genomic DNAs. Our results confirm the relatedness of C. tommasinianus, C. vittatus and C. heuffelianus at the Verni series of the Crocus genus. C. banaticus is taxonomically isolated as the sole member of the subgenus Crociris based on unique morphological features, but the difference is not convincing from AFLP data. The second interesting AFLP analysis result is the position of C. scepusiensis which separated it from the Crocus vernus aggregate.
Authors:H. Xie, Z. Lin, Z. Zhang, L. Du, Z. Xin, Y. Ma, X. Ye, and X. Chen
The common wheat line, YW243, developed in our research group, was tested for the resistances of barley yellow dwarf virus (BYDV), powdery mildew (Pm) and stripe rust in field, and was analyzed by molecular markers for convenient trace of the resistant genes in breeding. Genomic in situ hybridization (GISH) analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay further demonstrated that YW243 was a homozygous multiple translocation line of
Triticum aestivum, Thinopyrum intermedium
(T7DS·7DL-7XL & 1BL·1RS). The disease resistance test and marker analysis showed that YW243 carried seven resistance genes to the three diseases, including
to BYDV on 7DL-7XL,
to powdery mildew on 2AL,
Yr2, Yr9, Sr 31
and a new
to stripe rust on 7B, 1BL, 1RS and 2BL. Restriction fragment length polymorphism (RFLP) markers
, sequence tagged site (STS) marker STS
, simple sequence repeat (SSR) markers
, SSR markers
can be used as diagnostic tools to track
Bdv2, Pm4, Yr2, Yr9
, respectively; and two amplified fragment length polymorphism (AFLP) markers
can also be used to select
Authors:I. Karsai, P. Szűcs, K. Mészáros, K. Puskás, Z. Bedő, and O. Veisz
A barley mapping population consisting of 96 doubled haploid lines of anther culture origin was developed from the varieties Dicktoo and Kompolti Korai, which represent diverse types with respect to geographical origin and ecological adaptation. Several molecular marker techniques were used in mapping: among the markers with known chromosome location, RFLP, STS and SSR markers were applied to identify linkage groups and for comparative mapping, while RAPD and AFLP markers, which have random binding but provide useful information on polymorphism, were employed to fill in the linkage groups with markers. A total of 496 markers were tested in the DH population, 246 of which were included in the linkage map after eliminating markers that were completely linked with each other. The ratio of markers with known chromosome location to random markers in the 1107 cM map was one to three, and the mean recombination distance between the markers was 4.5 cM. Application of various marker methods and the effect of the population structure on the development of marker linkage maps are discussed.
Ajmone-Marsan, P., Castiglioni, P., Fusari, F., Kuiper, M., Motto, M.: 1998. Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLPmarkers. Theor. Appl. Genet., 96
Authors:M. Boczkowska, M. Harasimiuk, and A. Onyśk
Achleitner, A., Tinker, N., Zechner, E., Buerstmayr, H. 2008. Genetic diversity among oat varieties of worldwide origin and associations of AFLPmarkers with quantitative traits. Theor. Appl. Genet. 117 :1041