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AFLP Marker for Fusarium Head Blight Resistance in Wheat. In: Fedak, G., Choo, A. (eds), Proc. 2 nd Canadian Workshop on Fusarium Head Blight, 3–5 November 2001, Ottawa, Ontario, pp. 44–45.

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In industrial production of button mushroom (Agaricus bisporus) , the green mold epidemics by Trichoderma species cause serious damage. Sampling has preformed in various stages from pili, compost, seed and soil. Trichoderma selective media and PDA were used to isolate Trichoderma species. Out of 423 isolates, three sections of Trichoderma (Trichoderma, Pachybasium and Longibrachiatum) were identified.Among these isolates, more than 350 related to Trichoderma section, 35 to Pachybasium section and 20 related to Longibrachiatum section. T. harzianum, T. virens, T. atroviride, T. citrinoviride, T. ghanens and T. longibrachiatum were identified from Agaricus bisporus . No isolates of T. aggressivum f. aggressivum and T. aggressivum f. europaeum were obtained in this study. Agaricus bisporus is Matrix Nova for T. virens . The high-resolution fingerprinting method of amplified fragment length polymorphism (AFLP) analysis and sequencing of ITS regions were used to study the genetic relationships among Trichoderma isolates. A total of 1127 polymorphic AFLP loci were obtained using 20 primer combinations. No clear trend was detected between clustering in AFLP dendrogram and geographic origin of isolated materials. According to AFLP and morphological analysis, it is concluded that application of these markers resulted the same clustering and also genetic diversity in T. harzianum aggregate group proved byAFLP, suggesting thatAFLP is suitable marker along with sequencing of conserved regions for complementary classification of Trichoderma with other phylogenetic characteristics.

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Medini, M., Hamza, S., Rebai, A., Baum, M. 2005. Analysis of genetic diversity in Tunisian durum wheat cultivars and related wild species by SSR and AFLP markers. Genet. Res. Crop Evol. 52 :1–31. Baum M

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Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding molecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP fragments was followed in F2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit×El Haouaria, which had previously characterised by morphological and AFLP markers. A comparison of profiles from the morphologically contrasted parental individuals and F2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver-stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum forage crop.

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Crocus taxonomy has until now been based primarily on morphology, taking chromosome numbers into consideration. The genetics and genome structure of the genus, the relationships and diversity within the genus are not well known. Amplified fragment length polymorphism (AFLP) is a whole genome approach to study genetic variation that is gaining in popularity for lower-level systematics. The present study employed the AFLP technique for analyzing relationships among taxa of the Crocus genus (particularly the Crocus vernus aggregate) with Carpathian Basin origin. The molecular variance obtained was based on amplification, separation and detection of EcoRI and Tru1I double-digested Crocus spp. genomic DNAs. Our results confirm the relatedness of C. tommasinianus, C. vittatus and C. heuffelianus at the Verni series of the Crocus genus. C. banaticus is taxonomically isolated as the sole member of the subgenus Crociris based on unique morphological features, but the difference is not convincing from AFLP data. The second interesting AFLP analysis result is the position of C. scepusiensis which separated it from the Crocus vernus aggregate.

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The common wheat line, YW243, developed in our research group, was tested for the resistances of barley yellow dwarf virus (BYDV), powdery mildew (Pm) and stripe rust in field, and was analyzed by molecular markers for convenient trace of the resistant genes in breeding. Genomic in situ hybridization (GISH) analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay further demonstrated that YW243 was a homozygous multiple translocation line of Triticum aestivum, Thinopyrum intermedium and Secale cereale (T7DS·7DL-7XL & 1BL·1RS). The disease resistance test and marker analysis showed that YW243 carried seven resistance genes to the three diseases, including Bdv2 to BYDV on 7DL-7XL, Pm4 to powdery mildew on 2AL, Yr2, Yr9, Sr 31 and Lr26 and a new Yr to stripe rust on 7B, 1BL, 1RS and 2BL. Restriction fragment length polymorphism (RFLP) markers Xpsr687 and Xwg380 , sequence tagged site (STS) marker STS 1700 , simple sequence repeat (SSR) markers Xgwmc364 and Xgwm582 , SSR markers Xgwm388 and Xgwm501 can be used as diagnostic tools to track Bdv2, Pm4, Yr2, Yr9 and Yr in YW243 , respectively; and two amplified fragment length polymorphism (AFLP) markers M54E63 - 700 and M54E64 - 699 can also be used to select Yr in YW243 .

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A barley mapping population consisting of 96 doubled haploid lines of anther culture origin was developed from the varieties Dicktoo and Kompolti Korai, which represent diverse types with respect to geographical origin and ecological adaptation. Several molecular marker techniques were used in mapping: among the markers with known chromosome location, RFLP, STS and SSR markers were applied to identify linkage groups and for comparative mapping, while RAPD and AFLP markers, which have random binding but provide useful information on polymorphism, were employed to fill in the linkage groups with markers. A total of 496 markers were tested in the DH population, 246 of which were included in the linkage map after eliminating markers that were completely linked with each other. The ratio of markers with known chromosome location to random markers in the 1107 cM map was one to three, and the mean recombination distance between the markers was 4.5 cM. Application of various marker methods and the effect of the population structure on the development of marker linkage maps are discussed.

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Ajmone-Marsan, P., Castiglioni, P., Fusari, F., Kuiper, M., Motto, M.: 1998. Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers. Theor. Appl. Genet., 96

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Achleitner, A., Tinker, N., Zechner, E., Buerstmayr, H. 2008. Genetic diversity among oat varieties of worldwide origin and associations of AFLP markers with quantitative traits. Theor. Appl. Genet. 117 :1041

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AFLP markers. Theor. Appl. Genet. 98 :521–530. Jahoor A. Chromosome landing at the Mla locus in barley (Hordeum vulgare L.) by means of high-resolution mapping with AFLP markers

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