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Nitrogen (N) is an important nutrient for plant growth and yield production, and rice grown in paddy soil mainly uses ammonium (NH4 +) as its N source. Previous studies have shown that N status is tightly connected to plant defense; however, the roles of NH4 + uptake and assimilation in rice sheath blight disease response have not been studied previously. Here, we analyzed the effects of different N sources on plant defense against Rhizoctonia solani. The results indicated that rice plants grown in N-free conditions had higher resistance to sheath blight than those grown under N conditions. In greater detail, rice plants cultured with glutamine as the sole N source were more susceptible to sheath blight disease compared to the groups using NH4 + and nitrate (NO3 ) as sole N sources. N deficiency severely inhibited plant growth; therefore, ammonium transporter 1;2 overexpressors (AMT1;2 OXs) were generated to test their growth and defense ability under low N conditions. AMT1;2 OXs increased N use efficiency and exhibited less susceptible symptoms to R. solani and highly induced the expression of PBZ1 compared to the wild-type controls upon infection of R. solani. Furthermore, the glutamine synthetase 1;1 (GS1;1) mutant (gs1;1) was more susceptible to R. solani infection than the wild-type control, and the genetic combination of AMT1;2 OX and gs1;1 revealed that AMT1;2 OX was less susceptible to R. solani and required GS1;1 activity. In addition, cellular NH4 + content was higher in AMT1;2 OX and gs1;1 plants, indicating that NH4 + was not directly controlling plant defense. In conclusion, the present study showed that the activation of NH4 + uptake and assimilation were required for rice resistance against sheath blight disease.

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Journal of Thermal Analysis and Calorimetry
Authors: Imre Miklós Szilágyi, Eero Santala, Mikko Heikkilä, Marianna Kemell, Timur Nikitin, Leonid Khriachtchev, Markku Räsänen, Mikko Ritala, and Markku Leskelä

. Here we present a model study on the heat treatment of electrospun polyvinylpyrrolidone [PVP, (C 6 H 9 NO) n ] and ammonium metatungstate [AMT, (NH 4 ) 6 [H 2 W 12 O 40 ]· n H 2 O] nanofibers. It clearly demonstrates the importance of finding the

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Drug overdose is the most common method in suicide attempts. Amitriptyline (AMT), a tricyclic antidepressant agent, known for potentially lethal cardiovascular and neurological effects, is used especially by intoxicated patients. The aim of this study was to validate a method for the determination of AMT in gastric lavage samples by high-performance thin-layer chromatography (HPTLC). Artificial gastric lavage was used to obtain a better extraction procedure for AMT. The method was also applied to patients who attempted suicide with AMT. A validated, accurate, and rapid HPTLC-based method was developed for quantitation of AMT in gastric content of intoxicated patients. Extraction was done from 3 mL gastric lavage sample by liquid-liquid extraction procedure with ethyl acetate-n-heptane (1:1, w/w) in alkaline pH with 10.8. The mobile phase was an isocratic solvent system consisting of methanol-ammonia (25%) (98.5:1.5, v/v). 3,4-Methylenedioxy-Nmethamphetamine (MDMA) was used as internal standard (IS). Analytes were quantified by TLC Scanner operating under 209 nm. The retardation factors of AMT and MDMA were determined as 0.49 and 0.25, respectively. The analytical range was set as 10–250 ng spot−1 for AMT. Calibration was linear within the selected range in gastric lavage (r > 0.9995). Limit of detection and limit of quantification of AMT were found to be 5.1 and 17.3 ng spot−1, respectively. Recoveries of 25 ng spot−1 and 100 ng spot−1 concentrations were found 83.12% and 91.89%; bias% values were 10.91 and 3.19, respectively. The validated method was also applied on samples of five patients who attempted suicide by AMT, and concentrations in gastric lavage samples of 5 patients were found 7.1, 8.3, 9.9, 14.6, and 36.6 ng mL−1. This successfully validated method was applied to suicide cases, and it can be conveniently employed on both antemortem and postmortem cases suspected by amitriptyline.

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Abstract  

Cerebral amino acid metabolism is known to be associated with various psychosomatic disorders. 3-(123I)iodo-L-alpha methyl tyrosine (123I-L-AMT) is an alternative to the PET radiopharmaceutical, 11C- thymidine, for brain SPECT studies. Radioiodination of L-alphamethyl tyrosine using chloramine T as well as iodogen has been standardized using 125I as the first step towards the development of the SPECT imaging agent 123I-L-AMT. Purification of the iodinated product was carried out over Sephadex LH-20 column. A quick and easy purification method using Sep-pak column also has been standardized. Quality control of the 125I-L-AMT was carried out by estimating the radiochemical purity and stability of the product. Biodistribution studies of the product were carried out in mice. Time dependent pharmacokinetic studies and activity distribution pattern in the different parts of the brain were also carried out.

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Abstract  

In the present investigation, DTA and TG techniques were used to study the thermal behaviour of montmorillonite treated with solutions of the pesticide aminotriazole (AMT), in nitrogen flow. These techniques have been complemented by mass spectrometry of the evolved gases (EGA-MS). AMT is adsorbed in the interlamellar space of montmorillonite as a cation. Results obtained in this study show that this provokes a shift of the dehydroxylation peak of montmorillonite to lower temperatures than those of the untreated clay. Montmorillonite protects the adsorbedAMT, delaying its first decomposition step, and catalysesAMT final decomposition at lower temperatures. The DTA curve of montmorillonite-AMT mechanical mixture differs from the sum of those of the clay mineral and the pesticide heated individually. Montmorillonite dehydroxylation occurs at lower temperature, indicating a complex formation betweenAMT and the mineral during the heating process. However the DTA of the mixture is different from that of the complex previously studied, indicating that in the complex obtained by heating the physical mixtureAMT is adsorbed as neutral molecule or as a product of its decomposition.

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Anton Richter, ein gebürtiger Österreicher, erwarb im Sommer 1832 die Stelle als Regens Chori im Dom zu Győr. Als er im Oktober desselben Jahres sein Amt antrat, hat er als seine erste Aufgabe ein Inventar der existierenden Noten aufgestellt. Außerdem hat er eigenhändig eine Menge neuer Stimmen zu den Kompositionen abgeschrieben, wo die bisherigen Stimmen wegen des größeren Chor- und Orchester-bestandes nicht ausreichten. Werke der in Österreich wirkenden oder dort beliebten Komponisten, der sogenannten Kleinmeister ihrer Epoche, wurden um solche neuen Stimmen bereichert (z. B. Franz Aumann, Leopold Hofmann, Georg Reutter, Seiche bzw. Seuche, Franz Ignaz Tuma, Johann Georg Zechner und Gregor Joseph Werner). Im Dom zu Győr sind die Werke der Barockzeit erhalten geblieben.

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Absztrakt

Esetismertetés: A 35 éves nőbetegnél epigastrialis panaszok miatt végzett gastroscopia 2 cm-es antralis ulcust talált. Biopszia: közepesen differenciált intestinalis típusú adenocarcinoma. Praeoperativ staging CT és endoscopos UH: cT2cN0cM0. Műtét: Laparoscopos subtotalis gyomorresectio + módosított D2 lymphadenectomia, Roux-en-Y szerinti rekonstrukcióval. A duodenum és a gyomor mobilizálását LigaSure Dolphin (5 mm × 37 cm Covidien) segítségével végeztük. A proxymalis és distalis resectióhoz Endo-Gia Ultra Universal Stapler (Covidien) vágó-varró gépeket használtunk (60 mm-es AMT). Ezt követően módosított D2 lymphadenectomiát végeztünk a ligamentum hepatoduodenale, a truncus coeliacus, valamint az arteria lienalis eredésének környékéről. A side-to-side retrocolicus loop gastrojejunostomiát laparoscoposan Endo-Gia Ultra Universal Stapler segítségével készítettük el a gyomor hátsó falára. A felhozott jejunumkacsot Endo-Giával átvágtuk és lezártuk a gyomorcsonk resectiós vonalával egy szintben, a gyomorcsonk nyílását ugyanezzel a Gia-varrattal zártuk. Így a loop gastrojejunostoma Roux-en-Y anastomosissá alakítható. Az ‘Y’ end-to-side jejunojejunostomiát a specimen eltávolítására használt minilaparotomián kézi varrattal készítettük el. Műtéti idő: 200 perc, vérveszteség: 100 ml. Eszközök: Endo-Gia Ultra Universal Stapler vágó-varró gép + 6 darab 60 mm AMT‑tár + 1 darab 45 mm AMT-tár, LigaSure Dolphin 5 mm × 37 cm. A postoperativ szak eseménytelenül telt, a kilencedik ápolás napon a beteget panaszmentesen elbocsátottuk. Végleges szövettan: Invazív intestinalis típusú adenocarcinoma, 2,7 cm átmérő, pT1bpN0, HER-2 2+. Megbeszélés: A laparoscopos subtotalis gyomorresectio retrocolicus Roux-Y rekonstrukcióval onkológiai kompromisszum nélkül, jó funkcionális eredménnyel végezhető a gyomorrák korai stádiumaiban.

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Biologia Futura
Authors: Margit Kollaricsné Horváth, Borbála Hoffmann, István Cernák, Szilveszter Baráth, Zsolt Polgár, and János Taller

nitrate–reductase (NR), nitrite–reductase (NiR), ammonium transporter (AMT), and asparagine synthase (AS) genes was studied as described by Li et al. ( 2010 ). For reference, the cytochrome-oxidase I (coxI) was used. The qPCR experiment was performed in a

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Communal child care in Transylvania (Rumania) in the spirit of Saint Francis of Assisi

Primär-, Sekundär- und Tertiärprävention im Rahmen des von Csaba Böjte O.F.M. gegründeten Kinderschutznetzwerkes

European Journal of Mental Health
Author: Zsuzsanna Ládonyi

zur Bekämpfung der Diskriminierung in Beitritts- und Kandidatenstaaten (Luxemburg: Amt für amtliche Veröffentlichungen der Europäischen Gemeinschaften). Gleichstellung, Vielfalt und Erweiterung

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Richter B, Wilmes H, Falk R, Lothhammer A 1997: Absolute gravity measurements in Central and Eastern Europe. Measurement and analysis report, June 1997. Activity Report, Contract N. MF41G T0494 T0585, Amt für Militärisches Gewesen in Euskirchen and

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