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Lake Hévíz is the largest natural thermal lake of Europe, harboring special bacterial communities. The aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on Actinobacteria, by using cultivation-based and -independent molecular methods. Samples from two depths were taken in two different locations in October 2007. 245 strains were isolated, grouped to 85 OTUs by ARDRA, and identified by 16S rDNA sequencing. Most of the strains showed highest sequence similarity with Bacillus and related genera. Strains belonging to the phylum Actinobacteria were identified as members of Arthrobacter, Brachybacterium, Brevibacterium, Curtobacterium, Friedmanniella, Gordonia, Kocuria, Microbacterium, Micrococcus, Micromonospora, Mycobacterium, Rhodococcus, Streptomyces and Williamsia . Two clone libraries were constructed from H3M and H4M samples, providing 288 and 192 clones which were grouped to 150 and 125 OTUs, respectively, by ARDRA. The two most abundant group of the H4M library were OP8-related. The phylum Proteobacteria was represented mostly by δ -Proteobacteria, other relevant groups were Cyanobacteria, Bacteroidetes, Acidobacteria and β -Proteobacteria. The H3M library was dominated by Cyanobacteria, Verrucomicrobia, β -Proteobacteria, γ -Proteobacteria and δ -Proteobacteria. Chloroflexi, Bacteroidetes, Spirochetes and Firmicutes were scarce. Results from the clone libraries were compared to the length-heterogeneity-PCR fingerprints of the communities.

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A survey of current taxonomy of common foodborne bacteria

Part II. Gram-positive phyla of Firmicutes and Actinobacteria

Acta Alimentaria
Author:
T. Deák

Recent changes in classification of the four major groups of cultivable bacteria commonly encountered in foods are reviewed. Newly described species and genera as well as reassignment of former taxa belonging to Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria are considered. Taxonomic changes are derived from results of 16S rRNA gene analysis confirmed by other molecular techniques and traditional methods. The review is aimed to update relevant taxonomic information for those not directly involved in taxonomy, however, this kind of information will have significance in understanding the microbial ecology of food systems and promote improvement of preservation methods, fermentation technologies as well as enhance the safety of products.

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Allgaier, M. and H. P. Grossart. 2006. Diversity and seasonal dynamics of Actinobacteria populations in four lakes in Northeastern Germany. Appl. Environ. Microbiol. 72: 3489

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). Doi: http://dx.doi.org/10.1051/limn/2014023. 17. Hahn , M. W. : Description of seven candidate species affiliated with the phylum Actinobacteria, representing

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. Li , W.J. , Chen , H.H. , Kim , C.J. , Zhang , Y.Q. , Park , D.J. , Lee , J.C. , Xu , L.H. , Jiang , C.L. : Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the

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Acta Veterinaria Hungarica
Authors:
József Szabó
,
Gergely Maróti
,
Norbert Solymosi
,
Emese Andrásofszky
,
Tamás Tuboly
,
András Bersényi
,
Geza Bruckner
, and
István Hullár

,076 1,819 Actinobacteria 342 560 275 561 Ratios to Firmicutes Firmicutes/Bacteroidetes 1.05 0.51 1.10 0.48 Firmicutes/Verrucomicrobia 4.33 2.43 27.58 16.38 Firmicutes/Proteobacteria 4.80 3.59 13.54 4.83 Firmicutes/Actinobacteria 17.41 18.62 52.96 15

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Current taxonomy of common foodborne bacteria

Part I. Gram-negative phyla of proteobacteria and bacteroidetes

Acta Alimentaria
Author:
T. Deák

Recent changes in classification of the four major groups of cultivable bacteria commonly encountered in foods are reviewed. Newly described species and genera as well as reassignment of former taxa belonging to Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria are considered. Taxonomic changes are surveyed derived from results of 16S rRNA gene analysis confirmed by other molecular techniques and traditional methods. The review is aimed to update relevant taxonomic information for those not directly involved in taxonomy, however, this kind of information will have significance in understanding the microbial ecology of food systems and promote improvement of preservation methods, fermentation technologies as well as enhance the safety of products.

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Acta Microbiologica et Immunologica Hungarica
Authors:
Gergely Krett
,
Viktória Vágány
,
Judit Makk
,
Katalin Jáger
,
Mária Reskóné
,
Károly Márialigeti
, and
Andrea Borsodi

Lake Hévíz is the largest natural warm water lake of Europe. The curative mud of the lake comprises volcanic and marsh components although their species composition is hardly known yet. The aim of the present study was to gain information about the distribution and species diversity of bacterial communities inhabiting the sediment of Lake Hévíz using cultivation-based and molecular cloning methods. Samples from two depths and locations were taken in 2004 and 2007. Representatives of the altogether 255 bacterial isolates were affiliated with the phyla Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes. The most abundant groups belonged to the genus Bacillus (Firmicutes). Many of Lake Hévíz isolates showed the highest sequence similarity to bacteria known to be plant associated or members of normal human microbiota as well as participating in decomposition of highly resistant organic materials. In the three clone libraries, phylotypes belonging to altogether different phyla (Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes, Cyanobacteria, Chlorobi, Chloroflexi, Deferribacteres, Nitrospirae, Spirochaetes and Verrucomicrobia) were revealed from which members of Gammaproteobacteria and Cyanobacteria proved to be the most abundant. Regardless of the sampling times and methodology used, high spatial heterogeneities of bacterial community structures were characteristic of the sediment of Lake Hévíz.

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To analyze the rhizosphere bacterial communities in wetlands, the total lipid content was extracted from a peat soil and 4 abundant wetland plant roots ( Typha angustifolia L., Salix cinerea L., Carex pseudocyperus L., Thelypteris palustris Salisb.). The separated phospholipid fraction was further fractionated and deriva­tized prior to gas chromatography-mass spectrometry (GC-MS) measurement. In the evaluation only the bacteria-specific fatty acids were used in order to neglect fatty acid information derived from plant root cells. Based on these analyses, a high level bacterial concentration was demonstrated in the rhizosphere, and the relative occurrence of aerobe and anaerobe, Gram positive and negative bacteria, methanotrophs, sulphate reducers and Actinobacteria was determined. Through the PLFA analysis the study of bacteria regardless of culturability was possible.

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