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-01), adipogenesis (Gibco, A10070-01), and chondrogenesis differentiation medium (Gibco A10071-01), followed by incubation for 2 weeks with the induction medium replaced thrice a week. The staining procedure with Oil Red O, Alcian Blue and Alizarin Red S were done

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. (2010) Morphology of the stomach of the tropical house gecko Hemidactylus mabouia (Squamata: Gekkonidae). Acta Zoologica , 1–8. Scott, J. E. (1996) Alcian blue. Now you see it, now you don’t. Eur. J. Oral Sci

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Acta Biologica Hungarica
Authors: Joanna Klećkowska-Nawrot, Karolina Goździewska-Harłajczuk, Artur Kowalczyk, Ewa Łukaszewicz and Renata Nowaczyk

This study describes the macroscopic anatomy and the microscopic and ultrastructural features of the Harderian gland and lacrimal gland of the Capercaillies. It was conducted both on adult male and female Capercaillies. Tissue sections were stained with hematoxylin and eosin, azan trichrome, modified Mallory’s trichrome, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale’s dialysed iron. The morphometric study of the Harderian and lacrimal glands indicated that they are both larger in male than in female Capercaillies. The histological analysis showed that the HG has a multilobar tubulo-alveolar structure with numerous lymphocytes and plasma cells. The LG has a multilobar tubulo-acinar structure without lymphocytes and plasma cells. The periodic acid-Schiff staining and alcian blue pH 2.5 staining demonstrated a mild positive reaction in the epithelial cells of the Harderian gland and weak positive reaction in the lacrimal gland. The HDI staining detected the presence of carboxylated acid mucopolysaccharides in the Harderian and lacrimal glands. Transmission electron microscopy revealed the presence of two types of secretory vesicles in the cytoplasm of both studied glands. It also showed that lipid droplets and glycogen granules were more abundant in the Harderian gland than in the lacrimal gland of this species.

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Se describe la anatomía de la madera y de la corteza de un nuevo género y especie de Anacardiaceae: Attilaea abalak E. Martínez et Ramos. Las muestras se obtuvieron de la región de Calakmul, Campeche, México, en selva baja caducifolia con suelo calizo. Se prepararon cortes finos de madera y corteza utilizando un micrótomo de deslizamiento, los cuales fueron teńidos con azul alciano-safranina. Las características anatómicas se estudiaron en cortes transversales, tangenciales y radiales. Se encontraron diferencias significativas en las características anatómicas cuando se compararon con las de Spondias purpurea L., que es la especie más parecida morfológicamente que habita en sus cercanías. El análisis de las características anatómicas y el significativo hábito escandente de A. abalak llevan a concluir que se trata de un taxon diferente como lo señalan Martínez y Ramos (2007).

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Resolution and Discovery
Authors: Nada Žnidaršič, Polona Mrak, Eva Rajh, Kristina Žagar Soderžnik, Miran Čeh and Jasna Štrus

: (a) Evaluation of the cuticular matrix characteristics based on the staining by the selected histological/histochemical procedures, including hematoxylin–eosin, Alcian blue, and Methylene blue-Azure II staining; staining results comparison in non

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