Authors:Yonah H. Mwalwisi, Seraphina C. Omolo, Ludwig Hoellein, Danstan H. Shewiyo, Ulrike Holzgrabe and Eliangiringa Kaale
A simple, cost-effective, precise, accurate, and rapid planar chromatographic method was developed and validated for the separation and determination of amodiaquine and artesunate in tablet formulations. Both compounds were determined using high-performance thin-layer chromatography plates and a mobile phase composed of toluene, acetonitrile, methanol, ammonium acetate, and triethylamine in the ratio 10:5:3:1:0.5 (% v/v). Amodiaquine was evaluated densitometrically at a detection wavelength of λ = 345 nm, whereas artesunate was determined fluorimetrically at λ = 503 nm. The method was linear in the concentration ranges of 0.093–0.280 μg spot−1 for artesunate and 0.250–1.250 μg spot−1 for amodiaquine, respectively.
Authors:Shen Xuesong, Wang Tao, Jin Meihua, Zhao Chunxia, Qin Xuelian, Liu Hanfu, Qiu Zhuangping and Liu Yi
response, the detector produces a voltage which is proportional to the power output from the sample.
Standard artesunate drug was provided by Guilin Pharmaceutical Corp. Ltd. (Guo yao zhun zi H19994073
Authors:O. A. Adegoke, L. L. Xiang, O. S. Idowu and D.-Y. Chen
A highly sensitive and reproducible isocratic liquid chromatographic method has been developed for the analysis of artemisinin and its three commonly used derivatives (artesunate, dihydroartemisinin, and artemether). The method involves a precolumn derivatization reaction with 4-carboxyl-2,6-dinitrobenzene diazonium ion to produce azo adducts that are UV-active. The critical parameters for the derivatization such as temperature, reaction time, and reagent concentrations were studied and optimized. The chromatographic separations were carried out on a C-18 column with mobile phase consisting of acetonitrile-0.1% acetic acid (60:40) at a flow rate of 1 mL min−1. UV detection was set at 254 nm. Dynamic linear calibration range was obtained at concentrations of artemisinins ranging from 0.26 to 1.44 μg mL−1. The low limits of detections of artemisinin, artesunate, dihydroartemisinin, and artemether were found to be 0.091, 0.0125, 0.0489, and 0.0128 ng μL−1, respectively. The developed methods were precise (RSD <3%) and accurate (% error < 5%). The developed methods may find application in dosage form analysis and pharmacokinetic studies.
)–densitometry methods. The model process was applied earlier to formulations containing acetylsalicylic acid, acetaminophen, ibuprofen, and chlorpheniramine maleate [ 1 ]; mebendazole, diphenhydramine HCl, amodiaquine, and artesunate [ 2 ]; amodiaquine and diazepam [ 3
analyzed product (32.4 mg caffeine, 162 mg aspirin, and 110 mg acetaminophen; see Figure 2 ), was taken from a previously published method for the analysis of artesunate pharmaceutical products [ 7 ].