proteases is mainly performed by using Bacillus strains due to their several characteristics such as being non-pathogenic, easy to modify via genetic manipulations, low cost of production medium, extracellular production, short production time, and lacking
Fusarium wilt of tomato is one of the most prevalent and
economically important diseases of tomato worldwide especially in tropical
regions. The aims of the present study were to isolate and characterize
Bacillus bacteria from tomato rhizospheric soil of various
regions in Iran and determine the isolates that exhibit high levels of
antagonistic efficiency against tomato Fusarium wilt pathogen,
Fusarium oxysporum f. sp. lycopersici
(Fol) and growth promotion activity. In this study, 303
Bacillus isolates were obtained from tomato rhizospheric
soil. Dual culture and volatile metabolite tests were used to screen for
antagonism of Bacillus isolates against Fol.
Among them, 20 isolates were found to inhibit pathogen growth by 67.77% and
33.33% in dual culture and volatile metabolite tests, respectively. Based on the
results of physiological tests and 16S rRNA and gyrA gene
sequence analysis of 20 effective isolates, 11, seven and two isolates were
identified as B. subtilis, B. velezensis and
B. cereus, respectively. The results of greenhouse
assessment showed that KR1-2, KR2-7 and A2-9 isolates which were characterized
as Bacillus subtilis, reduced the disease index to 16.67% and
promoted the plant growth by 80%. These isolates may serve as potential
promising biocontrol agents against Fusarium wilt of
therapy. Then, if there is no need to pay attention to its safety while taking CUR or if there is any clause that we have to follow when it comes to drug combination?
Bacillus subtilis ( B. subtilis ), which is commonly found in soil and
Literature Ammouneh , H. , Harba , M. , Idris , E. and Makee , H. ( 2011 ): Isolation and characterization of native Bacillus Thuringiensis isolates from Syrian soil and testing under insecticidal activities against some insect pests
-Rivera, A., Benintende, G., Cozzi, J., Baizabal-Aguirre, V. M., Valdez-Alarcon, J. J., Lopez-Meza, J. E. (2004) Molecular characterization of
strains from Argentina.
Antonie Van Leeuwenhoek 86
In this study, bacterial biocontrol agents against the phytopathogen Lasiodiplodia theobromae were screened from Thua Nao, a Thai fermented soybean product. A total of 170 bacterial strains were isolated and initially screened for their antagonistic activity by dual culture test. Of these, 39 isolates were able to inhibit the fungal growth showing the percentage of inhibition ranging from 25.0–67.5. Among them, the isolate TN79 was selected as a potential antagonistic strain for further study. For this, the bacterial strain TN79 was cultured on nutrient agar for 2 weeks and its crude extracts were prepared using phosphate buffÍer pH 7.0. The bacterial crude extracts prepared were active and could inhibit all four fungal strains of L. theobromae. The optimum pH for antifungal activity of the extracts was 7. In addition, the extracts were also active when exposed to the UV light (254 nm) up to 1 hour and to proteinase K treatment (1 mg/ml). The bacterial strain TN79 was then characterized in terms of their phenotypic and genotypic properties including morphology, biochemical profiles, and rRNA gene sequence. Based on this analysis, the bacterium TN79 was closely related to Bacillus velezensis.