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Bittergourd yellow mosaic virus (BGYMV) is one of the virus affecting the bittergourd (Momordica charantia). It is transmitted by whitefly Bemisia tabaci Genn. Minimum of five whiteflies were required to transmit the virus. However, hundred per cent transmission of BGYMV disease was obtained when 45 whiteflies were released per plant. Twelve hours of acquisition access feeding period (AAFP) and inoculation access feeding period (IAFP) were required for the whiteflies to transmit the disease. The percentage of transmission increased with increase in both acquisition and inoculation feeding period. A pre-acquisition starvation period of 3.5 h ensured hundred per cent transmission, while post-acquisition starvation period affected the transmission.

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