Authors:Claudia Genger, Sigri Kløve, Soraya Mousavi, Stefan Bereswill and Markus M. Heimesaat
and subspecies [ 3 ]. Among these Campylobacter jejuni and Campylobactercoli are the most prevalent ones causing human morbidities. Campylobacter are widely distributed in the environment and can be found in a multitude of warm-blooded domestic
Authors:Oreva Ogbor, Abraham Ajayi, Andreas E. Zautner and Stella I. Smith
chicken and 2/8 (25%) of guinea fowl droppings. All 8 Campylobacter isolates were tested negative for the hip gene and thus classified as Campylobactercoli with a prevalence of 5.3% (8/150).
All C. coli isolates were resistant to one or
Campylobacter jejuni and C. coli are among the most important causes of acute diarrhoea in humans throughout the world. Poultry meat is a major source of Campylobacter infections. Sensitive detection methods are necessary to identify contaminated samples. Detection of campylobacters by culturing is slow and tedious, whereas PCR technology offers the potential for rapid and sensitive detection, however, it may be inhibited when used directly for food or pre-enriched food samples. Different methods for sample and/or DNA preparation were studied to find an optimal combination for sensitive PCR detection of C. coli in enrichment broth. Buoyant density centrifugation (BDC) prior to cell lysis improved PCR detection of C. coli by 100-1000-fold. Preston enrichment broth spiked with 101-102 CFU ml-1 was detected positive after 18 h of enrichment. Specific flaA PCR detection of C. coli in enrichment broth following BDC and simple heat lysis of the cells can be conducted within two working days. This study is a part of the undergoing development of a rapid and sensitive molecular procedure for specific detection of C. coli in foods.
Authors:Orsolya Erdősi, Katalin Szakmár, Zsuzsanna Szili, Géza Szita, Sándor Bernáth, József Sövényi and Péter Laczay
a selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B for the detection of Campylobacter jejuni and Campylobactercoli in chicken carcass rinses . Int. J. Food Microbiol. 162 , 308 – 310
Authors:Wycliffe Omurwa Masanta, Raimond Lugert, Uwe Groß, Gunter Linsel, Astrid Heutelbeck and Andreas Erich Zautner
J-H , Seo H-S , et al.: Antimicrobial susceptibility profiles and molecular typing of Campylobacter jejuni and Campylobactercoli isolates from ducks in South Korea . Appl Environ Microbiol 80 , 7604 – 7610 ( 2014 )