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and subspecies [ 3 ]. Among these Campylobacter jejuni and Campylobacter coli are the most prevalent ones causing human morbidities. Campylobacter are widely distributed in the environment and can be found in a multitude of warm-blooded domestic

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chicken and 2/8 (25%) of guinea fowl droppings. All 8 Campylobacter isolates were tested negative for the hip gene and thus classified as Campylobacter coli with a prevalence of 5.3% (8/150). All C. coli isolates were resistant to one or

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genotyping methods for Campylobacter jejuni and Campylobacter coli . J. Clin. Microbiol. 38 , 1940–1946. Wagenaar J. A. Computer-assisted analysis and epidemiological value

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Campylobacter jejuni and C. coli are among the most important causes of acute diarrhoea in humans throughout the world. Poultry meat is a major source of Campylobacter infections. Sensitive detection methods are necessary to identify contaminated samples. Detection of campylobacters by culturing is slow and tedious, whereas PCR technology offers the potential for rapid and sensitive detection, however, it may be inhibited when used directly for food or pre-enriched food samples. Different methods for sample and/or DNA preparation were studied to find an optimal combination for sensitive PCR detection of C. coli in enrichment broth. Buoyant density centrifugation (BDC) prior to cell lysis improved PCR detection of C. coli by 100-1000-fold. Preston enrichment broth spiked with 101-102 CFU ml-1 was detected positive after 18 h of enrichment. Specific flaA PCR detection of C. coli in enrichment broth following BDC and simple heat lysis of the cells can be conducted within two working days. This study is a part of the undergoing development of a rapid and sensitive molecular procedure for specific detection of C. coli in foods.

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Evaluation of an enzyme immunoassay-based stool antigen test to detect Campylobacter jejuni and Campylobacter coli Diagn Microbiol Infect Dis 58 171 175

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541 Frost, J. A., Oza, A. N., Thwaites, R. T., Rowe, B.: Serotyping scheme for Campylobacter jejuni and Campylobacter coli based on direct agglutination of heat-stable antigens. J Clin

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Authors: Orsolya Erdősi, Katalin Szakmár, Zsuzsanna Szili, Géza Szita, Sándor Bernáth, József Sövényi and Péter Laczay

a selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B for the detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinses . Int. J. Food Microbiol. 162 , 308 – 310

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Authors: Wycliffe Omurwa Masanta, Raimond Lugert, Uwe Groß, Gunter Linsel, Astrid Heutelbeck and Andreas Erich Zautner

J-H , Seo H-S , et al.: Antimicrobial susceptibility profiles and molecular typing of Campylobacter jejuni and Campylobacter coli isolates from ducks in South Korea . Appl Environ Microbiol 80 , 7604 – 7610 ( 2014 ) 11

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Authors: Norah Lynn-Anne Mund, Wycliffe Omurwa Masanta, Anne-Marie Goldschmidt, Raimond Lugert, Uwe Groß and Andreas E. Zautner

distinguish Campylobacter jejuni from Campylobacter coli . BMC Microbiol 14 , 262 ( 2014 ) 8. Marchant J , Wren B , Ketley J : Exploiting genome

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111 245 255 Kokotovic, B. and On, S. L. W. (1999): High-resolution genomic fingerprinting of Campylobacter jejuni and Campylobacter coli by

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