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Orvosi Hetilap
Authors: János Megyesi, Anna Biró, László Wigmond, Jenő Major, and Anna Tompa

Valverde, M., Ostrosky-Wegman, P., Rojas, E., et al.: The application of single cell gel electrophoresis or comet assay to human monitoring studies. Salud Pública Méx., 1999, 41 (Suppl.2), S109–S113

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Acta Biologica Hungarica
Authors: Gabrielle Souza Rocha, A. Fonseca, Michelle Rodrigues, F. Dantas, A. Caldeira-de-Araujo, and R. Santos

Ward, T. H., Butler, J., Shahbakhti, H., Richards, J. (1997) Comet assay studies on the activation of two diaziridinylbenzoquinones in K562 cells. Biochem. Pharmacol. 53 , 1115–1121. Richards J

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Biologia Futura
Authors: Rubina Tu¨nde Szabó, Mária Kovács-Weber, Márta Erdélyi, Krisztián Balogh, Natasa Fazekas, Ákos Horváth, Miklós Mézes, and Balázs Kovács

et al., 2012 ). The study of Hafner et al. ( 2012 ) showed the migration of DNA in the spleen of roosters after 17-day-long T-2 toxin exposure. Less than 1.5 mg of T-2 toxin/kg feed DNA breaking was not visible by the comet assay in leukocytes

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Acta Veterinaria Hungarica
Authors: Katalin Horvatovich, Dóra Hafner, Zsófia Bodnár, Gergely Berta, Csaba Hancz, Mike Dutton, and Melinda Kovács

Bony, S., Carcelen, M., Olivier, L. and Devaux, A. (2006): Genotoxicity assessment of deoxynivalenol in the Caco-2 cell line model using the comet assay. Toxicol. Lett. 166 , 67–76. Devaux A

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Abstract  

DNA Comet Assay method was carried out to detect irradiation treatment of some foods like meat, spices, beans and lentils. The fresh meat of cow and duck were irradiated up to radiation doses of 3 kGy, the spices (cardamoms and cumin black) were irradiated to radiation doses of 5, 10, 15 and 20 kGy while the beans (black beans and white beans) and lentils (red and green lentils) were irradiated to 0.5 and 1 kGy. All the foods were then analyzed for radiation treatment using simple microgel electrophoresis of single cells or nuclei (DNA Comet Assay). Sedimentation, lysis and staining times were adjusted to get optimized conditions for correct and easy analysis of each food. Using these optimized conditions, it was found out that radiation damaged DNA showed comets in case of irradiated food samples, whereas in non-treated food samples, round or conical spots of stained DNA were visible. Shape, length and intensity of these comets were also radiation dose dependent. Screening of unirradiated and irradiated samples by Comet Assay was successful in the case of all the foods under consideration under the optimized conditions of assay. Therefore, for different kinds of irradiated foods studied in the present study, the DNA Comet Assay can be used as a rapid, simple and inexpensive screening test.

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Abstract  

The sulphur-containing proteinaceous amino acids l-cysteine, l-cystine and l-methionine were irradiated in the solid state to a dose of 3.2 MGy. This dose corresponds to that delivered by radionuclide decay in a timescale of 1.05 × 109 years to the organic matter buried at a depth >20 m in comets and asteroids. The purity of the sulphur-containing amino acids was studied by differential scanning calorimetry (DSC) before and after the solid state radiolysis and the preservation of the chirality after the radiolysis was studied by chirooptical methods (optical rotatory dispersion, ORD) and by FT-IR spectroscopy. Although the high radiation dose of 3.2 MGy delivered, all the amino acids studied show a high radiation resistance. The best radiation resistance was offered by l-cysteine. The radiolysis of l-cysteine leads to the formation of l-cystine. The radiation resistance of l-methionine is not at the level of l-cysteine but also l-methionine is able to survive the dose of 3.2 MGy. Furthermore in all cases examined the preservation of chirality after radiolysis was clearly observed by the ORD spectroscopy although a certain level of radioracemization was measured in all cases. The radioracemization is minimal in the case of l-cysteine and is more pronounced in the case of l-methionine. In conclusion, the study shows that the sulphur-containing amino acids can survive for 1.05 × 109 years and, after extrapolation of the data, even to the age of the Solar System i.e. to 4.6 × 109 years.

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Acta Biologica Hungarica
Authors: Xiang-Rong Xu, Fu-Qing Tan, Jun-Quan Zhu, Ting Ye, Chun-Lin Wang, Yi-Feng Zhu, Hans-Uwe Dahms, Fan Jin, and Wan-Xi Yang

Giovannelli, L., Decorosi, F., Dolara, P., Pulvirenti, L. (2003) Vulnerability to DNA damage in the aging rat Substantia nigra : a study with the comet assay. Brain Res. 969 , 244–247. Pulvirenti L

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Acta Biologica Hungarica
Authors: D. Đikić, Snježana Židovec-Lepej, Anica Remenar, Anica Horvat-Knežević, Vesna Benković, D. Lisičić, Lana Sajli, and O. Springer

testing, Results of the in vivo Comet assay workgroup. Mutat. Res. 627 , 31–35. Hartmann A. Fourth international Workgroup on Genotoxicity testing, Results of the in vivo Comet

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equos declinis mitigat aestas (Buc . 1, 1) Egybevág az összehasonlításban megjelenített kártékony hatású üstökös leírása is: Quantus conspicuo rutilans fulgore cometes nuntiat horrenda bella cruenta iuba. (Eleg . 22, 13–14) Numquid utrumque polum, sicut

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174 Lee, R. F., Steinert, S. (2003) Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and fresh water) animals. Mutat. Res. 544 , 43

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