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Introduction Cordyceps sinensis , a famous traditional Chinese medicine, is a parasitic fungus growing on the larvae of Hepialidae caterpillar. It has been used to tonify the kidney, replenish the lung, stanch bleeding, and resolve phlegm for

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–2), 27–29. 22 Liu, Y. K., Shen, W.: Inhibitive effect of Cordyceps sinensis on experimental hepatic fibrosis and its possible mechanism. World J. Gastroenterol

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Abstract

An ultra-rapid analytical method for determination of andrographolide and dehydroandrographolide in Andrographis Herba (AH) was developed by liquid chromatography with mass spectrometry (LC-MS). The sample was ultrasonically extracted with 10 mL 40% (v/v) methanol, and then purified with a C18 solid phase extraction column. The LC separation was performed on a Poroshell 120 EC-C18 column (30 × 2.1 mm, 2.7 μm) and eluted with 0.5 mmol L−1 ammonium acetate aqueous solution and acetonitrile (65:35) at a flow rate of 0.7 mL min−1, and detected by mass spectrometry (MS). The LC-MS analytical time was less than 1 min. The new developed method presented a good linearity (r > 0.9900), precision and repeatability (RSD < 2.0%). The recoveries for andrographolide and dehydroandrographolide were 93.5% (RSD = 2.2%) and 97.7% (RSD = 2.4%), respectively. The developed method was successfully applied in determination of andrographolide and dehydroandrographolide in seven batches of AH samples, and the contents of analytes in all samples were complied with the relative acceptance criteria in Chinese Pharmacopeia (>0.8%). This new developed LC-MS method is an ultra-rapid assay method for AH, which will help to improve the efficiency and reduce the cost of AH sample test.

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A simple, rapid, and effective high-performance thin-layer chromatographic (HPTLC) method has been established for differentiating among the polysaccharides present in six traditional Chinese medicines (TCM), Cordyceps sinensis, Ganoderma lucidum, Astragalus memberanaceus, Panax ginseng, Panax quinquefolii , and Panax notogiseng . Acid hydrolyzates of the polysaccharides were analyzed by HPTLC with two detection reagents, aniline-diphenylamine-phosphoric acid and ninhydrin, and scanning densitometry. The compounds were separated on silica gel plates with chloroform- n -butanol-methanol-acetic acid-water 4.5:12.5:5:1.5:1.5 ( v/v ) as mobile phase. Seven monosaccharides and two glucuronic acids were used as reference compounds. The results showed that hydrolysis of polysaccharides can release specific molecules present in the herbal species in addition to the monosaccharides present. This is useful for distinguishing the origins of the polysaccharides in Chinese medicines.

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] Cordyceps militaris chicken soup Enzymolysis promoted the volatilization of typical flavor compounds, cordyceps militaris inhibited some flavor substances [ 33 ] Iberian ham Identified the feeding methods and classified the samples [ 34 ] Fruit and

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2009 Antifungal and anticancer activities of a protein from the mushroom Cordyceps militaris Korean J Physiol Pharmacol 13 49 54

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. Chemical composition and bioactivity changes in stale rice after fermentation with Cordyceps sinensis . J. Biosci. Bioeng. 2008 , 106 ( 2 ), 188 – 193 . 10.1263/jbb.106.188 9. Folch-Fortuny , A. ; Prats-Montalbán , J. M. ; Cubero , S. ; Blasco

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