]. Furthermore, researchers have reported that many plants extract possessed antimicrobial activity against S. aureus [ 7 , 8 ]. Curcumin (diferuloylmethane)-(1,7-bis (4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) is a natural compound isolated from the
Authors:Hayun Hayun, Rina Rahmawati, Yahdiana Harahap, and Santi Purna Sari
Curcumin, commonly called diferuloylmethane (Figure 1 ), is the major coloring agent of turmeric ( Curcuma longa L.) and javanese turmeric or temulawak ( Curcuma xanthorrhiza Roxb). Curcumin has various
A simple, precise, rapid, selective, and cost-effective high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous estimation of curcumin, piperine, and thymol in an ayurvedic formulation. Chromatography was performed on silica gel 60 F
plates with toluene-ethyl acetate-methanol, 9 + 1 + 0.5 (
), as mobile phase. Plates were developed to a distance of 8 cm at room temperature, without chamber saturation. The plates were scanned and the compounds were quantified at their wavelengths of maximum absorption, 420, 333, and 277 nm for curcumin, piperine, and thymol, respectively. The respective
values of curcumin, piperine, and thymol were 0.23, 0.30, and 0.64. Response was a linear function of the amount applied to the plate in the ranges 50–250 ng, 10–60 ng, and 100–700 ng for curcumin, piperine, and thymol, respectively. LOD for curcumin, piperine, and thymol were 25, 5, and 50 ng, respectively. The mean results from assay of curcumin, piperine, and thymol in the ayurvedic formulation were found to be 0.85, 12.93, and 3.29 mg g
, respectively. The respective covariance for curcumin, piperine, and thymol was 0.78, 0.51, and 0.69%, respectively. Recovery was 100.41, 99.52, and 101.21% for curcumin, piperine and thymol, respectively. Rapid identification of curcumin, piperine, and thymol is also possible by spraying the plate with anisaldehyde in sulfuric acid reagent.
Authors:Zhao Namula, Yoko Sato, Manita Wittayarat, Quynh Anh Le, Nhien Thi Nguyen, Qingyi Lin, Maki Hirata, Fuminori Tanihara, and Takeshige Otoi
beneficial effects and improved the production system of porcine embryos. Curcumin, a common dietary pigment and spice, is a potent antioxidant and anti-inflammatory agent extracted from turmeric ( Curcuma longa ), and is known to play an essential role
, Lin SY, Su CC, Lin SS, Ho CC, Hsia TC, Yu CS, Ip SW, Lin TP, Chung JG: Effects of curcumin on N-bis(2-hydroxypropyl) nitrosamine (DHPN)-induced lung and liver tumorigenesis in BALB/c mice in vivo. In Vivo 22(6), 781–785 (2008
Authors:Shuanghu Wang, Zixia Lin, Ke Su, Jing Zhang, Lijing Zhang, Zhimou Gao, Zhiyi Wang, Jianshe Ma, and Xianqin Wang
worked, prolonging survival time of paraquat-induced animals and slowing down the damage of the lung [ 18 , 19 ], such as curcumin and pirfenidone. Though there are some successful animal experiments, they are not supported in terms of clinical practice
Authors:S. Mollayi, S. Tamhidi, H. Hashempour, and A. Ghassempour
Curcumin is the main bioactive constituent of turmeric rhizome. A wide range of pharmacological activities of curcumin has been reported. There has been growing interest in the development of preparative purification of curcumin. The objective of this study is the isolation of curcumin from other components by using recycling preparative HPLC method from curcuma longa L. The extraction was achieved by using ultrasonic system with three different solvents, including acetone, dichloromethane, and ethanol. After optimization of extraction process, preparative HPLC system was first applied for separation of curcumin. Because of poor resolution of preparative HPLC, recycled preparative HPLC was applied for separation of curcumin with higher purity up to five cycles. Recycled preparative HPLC resulted in improvement in separation factors which led to the increase of curcumin purity up to 99.5%. This method is simple, highly efficient, environmentally friendly, and has been demonstrated to be effective for preparation and purification of curcumin from curcuma longa L.
Curcumin (CUR) is extensively distributed in curcuma genus, and it attracts many people in the world by virtue of its special color and taste [ 1 , 2 ]. CUR has been introduced as a food additive in India, China