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Svanholm, C., Bandholtz, L., Castaños-Velez, E., Wigzell, H., Rottenberg, M. E.: Protective DNA immunization against Chlamydia pneumoniae . Scand. J. Immunol. 51 , 345–353 (2000). Rottenberg M. E

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Direct DNA inoculations were used to determine the efficacy of gene immunisation of chickens to elicit protective immune responses against infectious bursal disease virus (IBDV). Thevp2 gene of IBDV strains GP40 and D78, and thevp2-vp4-vp3 encoding segment of strain D78 were cloned in an expression vector which consisted of human cytomegalovirus (HCMV) immediate early enhancer and promoter, adenovirus tripartite leader sequences and SV40 polyadenylation signal. For purification of vaccine-quality plasmid DNA fromE. coli, an effective method was developed. Chickens were vaccinated by inoculation of DNA by two routes (intramuscular and intraperitoneal). Two weeks later, chickens were boosted with DNA, and at 2 weeks post-boost, they were challenged with virulent IBDV strain. Low to undetectable levels of IBDV-specific antibodies and no protection were observed with DNA encoding VP2. However, plasmids encoding VP2-VP4-VP3 induced IBDV-specific antibodies and protection in the chickens. DNA immunisation opens a new approach to the development of gene vaccines for chickens against infectious diseases.

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Acta Microbiologica et Immunologica Hungarica
Authors: Ildikó Faludi, Ágnes Szabó, Katalin Burián, Valéria Endrész and A. Miczák

alteration of Mycobacterium smegmatis to improve mycobacterium-mediated transfer of plasmid DNA into mammalian cells and DNA immunization. Infect. Immun. 75 , 4804–4816 (2007). Fennelly G. J

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., Begoyan, G., Yu, J., Glenn, G. M., Ross, T. M., Agadjanyan, M. G., Cribbs, D. H.: Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization. DNA Cell Biol. 27 , 19–24 (2008

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27 3267 3270 Repique, C. J., Li, A., Collins, F. M., Morris, S. L.: DNA immunization in a mouse model of latent tuberculosis: Effect of DNA

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immune response. This result suggests a positive role for LC3 in enhancement of CD4 + Th1 immune response. On the other hand, as we expected, CD8 + T cell responses were not increased in LC3-fused multi-epitope DNA immunized group compared with multi

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