Authors:Nadja Fodor, S. K. Dube, I. Fodor, E. Horváth, Edith Nagy, V. N. Vakharia and Altancsimeg Rencendorsh
Direct DNA inoculations were used to determine the efficacy of gene immunisation of chickens to elicit protective immune responses against infectious bursal disease virus (IBDV). Thevp2 gene of IBDV strains GP40 and D78, and thevp2-vp4-vp3 encoding segment of strain D78 were cloned in an expression vector which consisted of human cytomegalovirus (HCMV) immediate early enhancer and promoter, adenovirus tripartite leader sequences and SV40 polyadenylation signal. For purification of vaccine-quality plasmid DNA fromE. coli, an effective method was developed. Chickens were vaccinated by inoculation of DNA by two routes (intramuscular and intraperitoneal). Two weeks later, chickens were boosted with DNA, and at 2 weeks post-boost, they were challenged with virulent IBDV strain. Low to undetectable levels of IBDV-specific antibodies and no protection were observed with DNA encoding VP2. However, plasmids encoding VP2-VP4-VP3 induced IBDV-specific antibodies and protection in the chickens. DNA immunisation opens a new approach to the development of gene vaccines for chickens against infectious diseases.
Authors:J. Ongrádi, B. Stercz, Valéria Kövesdi and L. Vértes
., Begoyan, G., Yu, J., Glenn, G. M., Ross, T. M., Agadjanyan, M. G., Cribbs, D. H.: Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNAimmunization. DNA Cell Biol.
, 19–24 (2008
Authors:Jale Moradi, Maryam Izad, Mina Tabrizi, Nader Mosavari, Behnaz Esmaeili and Mohammad Mehdi Feizabadi
immune response. This result suggests a positive role for LC3 in enhancement of CD4 + Th1 immune response. On the other hand, as we expected, CD8 + T cell responses were not increased in LC3-fused multi-epitope DNAimmunized group compared with multi