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Abstract  

DNA adsorbed very low amount of water at low relative humidities, amount of adsorption increased to 140% at 98% relative humidity at 25°C. Heat of adsorption was 109 kJ mol-1 H2O for the increase of moisture content from 0 to 1.96%. At higher moisture contents the heat released approached heat of condensation of water vapour on free liquid surface, 40 kJ mol-1 H2O.

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JPC - Journal of Planar Chromatography - Modern TLC
Authors: Mitja Križman, Jernej Jakše, Mirko Prošek, Dea Baričevič and Branka Javornik

Agarose gel electrophoresis is a basic separation tool used in molecular biology, mostly for qualitative DNA analysis. There are constraints limiting its use in quantitative analysis, namely low repeatability and a narrow linear range. However, by using an internal standard or internal normalization, repeatability and linear range could be significantly improved. In the work discussed in this paper it was shown that an approximately fivefold improvement in repeatability and an over threefold wider linear range could be achieved by applying internal normalization. Using the proposed approach, genetic markers, for example RAPD and PCR-RFLP, or even microsatellite markers, could be conveniently quantitatively assessed using agarose gel electrophoresis.

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Interventional Medicine and Applied Science
Authors: Balázs Nemes, É. Toronyi, K. Rajczy, A. Szakos, B. Somlai, A. Doros, R. Chmel, F. Derner and L. Kóbori

. L. Keresztury K. Rajczy T. Tauszik 2003 DNA typing revealing high HLA

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Abstract  

Equilibrium dialysis and atomic absorption analysis were used to obtain adsorption isotherms and determine the stoichiometric binding constants of Cu(II) and Cd(II) ions to DNA from Spirulina platensis in solutions. The stoichiometric constants of Cu(II) and Cd(II) ions with DNA from S. platensis in 3 mM NaCI are 15.56⋅104 and 14.40⋅104, respectively. Effect of ionic strength and DNA GC content on binding constants of Cu(II)- and Cd(II)-DNA complexes were studied out. It was showed that the binding constants of Cu(II)- and Cd(II)-DNA complexes decrease with increase of ionic strength. The empirical dependences of logK on the GC content has been derived for Cd(II)- and Cu(II)-DNA complexes.

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Abstract  

By method of differential scanning calorimetry the absolute values of heat capacity for the systemwater–biopolymer (globular and fibrillar proteins and DNA) were measured in a wide range of temperatures (from -30 up to 130°C) and concentrations of proteins both in native and denatured states. Thermal properties of humid denatured biopolymers demonstrate a characteristic anomaly in the form of the heat capacity jump at temperature depending on the bound water content. It has been shown that in the systems studied a glass transition, where water serves as a native plasticizer, is observed. It has been established that the S-shaped character of all heat capacity curves obtained on dehydration for native and denatured biopolymers is due to the gradual transition to the glassy state of both native and denatured samples. It was found that thermally denatured humid small globular proteins at subsequent dissolving in water at room temperature are able to restore their native structure.

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One of the plasmids present in a Haemophilus somnus strain isolated from nasal discharge of a cattle with respiratory disease was purified and cloned for DNA sequencing. The plasmid was found to be 1065 base pairs long with 39.2% G+C content, and showed no homology to any DNA sequenced so far. It has no capacity to code any protein longer than 43 residues. It is not clear yet if this plasmid codes Haemophilus somnus specific factors.

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Acta Alimentaria
Authors: J. Goon, C. Choor, R. Ainaa, X. Sze, M. Syahriah, M. Syamimi, S. Rashidi, M. Mardiyanna and J. Zakiah

1732 1735 Sing , N.P., Stephen , R.E. & Schneider , E.L. (1988): A simple technique for quantitation of low levels of DNA damage in individual cells. Expl Cell

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Journal of Thermal Analysis and Calorimetry
Authors: G. Mrevlishvili, A. Carvalho, M. Ribeiro da Silva, T. Mdzinarashvili, G. Razmadze and T. Tarielashvili
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DNA by the use of activated charcoal during DNA extraction . – Plant Breeding . 115 : 205 – 206 . https://doi.org/10.1111/j.1439-0523.1996.tb00905.x Brondmann , P

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Journal of Thermal Analysis and Calorimetry
Authors: Jamlet R. Monaselidze, Maya T. Kiladze, Maya Z. Gorgoshidze, David G. Khachidze, Vasil G. Bregadze, Eteri M. Lomidze and Theimuraz A. Lezhava

Introduction During the past decade, a significant progress has been achieved in synthesis of new small molecules that can bind to DNA at selected sites, and can interfere with transcription, DNA replication, and reparation

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