DNA adsorbed very low amount of water at low relative humidities, amount of adsorption increased to 140% at 98% relative humidity
at 25°C. Heat of adsorption was 109 kJ mol-1 H2O for the increase of moisture content from 0 to 1.96%. At higher moisture contents the heat released approached heat of condensation
of water vapour on free liquid surface, 40 kJ mol-1 H2O.
Authors:Mitja Križman, Jernej Jakše, Mirko Prošek, Dea Baričevič and Branka Javornik
Agarose gel electrophoresis is a basic separation tool used in molecular biology, mostly for qualitative DNA analysis. There are constraints limiting its use in quantitative analysis, namely low repeatability and a narrow linear range. However, by using an internal standard or internal normalization, repeatability and linear range could be significantly improved. In the work discussed in this paper it was shown that an approximately fivefold improvement in repeatability and an over threefold wider linear range could be achieved by applying internal normalization. Using the proposed approach, genetic markers, for example RAPD and PCR-RFLP, or even microsatellite markers, could be conveniently quantitatively assessed using agarose gel electrophoresis.
dialysis and atomic absorption analysis were used to obtain adsorption isotherms
and determine the stoichiometric binding constants of Cu(II) and Cd(II) ions
to DNA from Spirulina platensis in solutions.
The stoichiometric constants of Cu(II) and Cd(II) ions with DNA from S. platensis in 3 mM NaCI are 15.56⋅104
and 14.40⋅104, respectively.
of ionic strength and DNA GC content on binding constants of Cu(II)- and Cd(II)-DNA
complexes were studied out. It was showed that the binding constants of Cu(II)-
and Cd(II)-DNA complexes decrease with increase of ionic strength. The empirical
dependences of logK on the GC content has been derived for Cd(II)- and Cu(II)-DNA
Authors:G. Tsereteli, T. Belopolskaya, N. Grunina and O. Vaveliouk
By method of differential scanning calorimetry the absolute values of heat capacity for the systemwater–biopolymer (globular
and fibrillar proteins and DNA) were measured in a wide range of temperatures (from -30 up to 130°C) and concentrations of
proteins both in native and denatured states. Thermal properties of humid denatured biopolymers demonstrate a characteristic
anomaly in the form of the heat capacity jump at temperature depending on the bound water content. It has been shown that
in the systems studied a glass transition, where water serves as a native plasticizer, is observed. It has been established
that the S-shaped character of all heat capacity curves obtained on dehydration for native and denatured biopolymers is due
to the gradual transition to the glassy state of both native and denatured samples. It was found that thermally denatured
humid small globular proteins at subsequent dissolving in water at room temperature are able to restore their native structure.
Authors:R. Izadpanah, Á. Dán, Mária Benkő, M. Rusvai, L. Fodor and B. Harrach
One of the plasmids present in a Haemophilus somnus strain isolated from nasal discharge of a cattle with respiratory disease was purified and cloned for DNA sequencing. The plasmid was found to be 1065 base pairs long with 39.2% G+C content, and showed no homology to any DNA sequenced so far. It has no capacity to code any protein longer than 43 residues. It is not clear yet if this plasmid codes Haemophilus somnus specific factors.
Authors:Jamlet R. Monaselidze, Maya T. Kiladze, Maya Z. Gorgoshidze, David G. Khachidze, Vasil G. Bregadze, Eteri M. Lomidze and Theimuraz A. Lezhava
During the past decade, a significant progress has been achieved in synthesis of new small molecules that can bind to DNA at selected sites, and can interfere with transcription, DNA replication, and reparation