The amorphous content of different Desferal samples was quantified by recording its recrystallization using isothermal microcalorimetry in a static as well as in a flowing humid atmosphere. Furthermore water vapor sorption gravimetry was performed for the same purpose. These analytical methods result in a quantitative signal directly dependent on the content of the amorphous phase (recrystallization, water sorption equilibrium). Their sensitivity allows the detection of amorphous content below 1%. Methods are compared and advantages and disadvantages are discussed.
A simple and sensitive spectrophotometric method for the determination of cobalt, nickel and copper with desferrioxamine-B (desferal) is described. The sensitivity of the colour reactions of cobalt, nickel and copper is increased by introducing desferal. The pH range for the formation of Co II, Ni II or Cu II-desferal. Chelates is 4.5–8.5, 5.5–9, and 6.5–9.5, respectively. The molar absorptivities of the chelates are 1.2·105, 5.6·104 and 4.8·104 at 545 nm, 390 nm and 680 nm, respectively. Beer's law is obeyed up to 10 g/ml of metal ion. The effect of desferal concentration, pH, standing time and interfering ions are discussed. The method was applied to the determination of trace amounts of cobalt, nickel and copper in steel and simulated radioactive wastes.
Authors:N. Balogh, F. Krausz, P. Lévai, and et al.
Morehouse, K. M., Flitter, W. D. and Mason, R. P. (1987): The enzymatic oxidation of Desferal to a nitroxide free radical. FEBS Lett. 22 , 246-250.
The enzymatic oxidation of Desferal to a nitroxide free radical