the body and injured gastrointestinal function, it is particularly appropriate to use pre-digested proteins, where pre-hydrolysis mimics the gastrointestinal digestion processes. Food containing peptides does not cause such hypertension as free amino
germination by action of various hydrolytic enzymes also results in improving the in vitro starch and protein digestibility and absorption characteristics compared to non-germinated ones ( Zhang et al., 2014 ). Biochemical activities taking place during
The aim of the study was to compare the effect of pressure and microwave cooking on the in vitro protein digestibility of bean seeds
. The results of the in vitro digestibility ascertained the improvement of protein digestibility affected by pressure-cooking of seeds. The digestibility of proteins of microwave-cooked bean seeds was lower. The electrophoretic SDS-PAGE separation patterns of bean proteins hydrolysed with trypsin indicated a significant influence of both treatments on the proteins examined. Degradation of proteins was apparent, however, the dominant fraction of 47–41 kDa remained intact, which confirms its resistance to digestion.
The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A
sp. dominated the thermophilic, while a
sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.
A calorimetric procedure is developed to study the effect on the soil of the effluents resulting for the anaerobic digestion
of slaughtering houses residues. DSC was used to study the pyrolysis properties of the effluent and the soil while isothermal
calorimetry is applied to study the microbial activity in the effluent and to assess on its effect on the microbial activity
of the soil where the industrial digester will be situated. The calorimetric data were studied together with the chemical
and biological properties of that residue. Results showed that effluent is constituted by low levels of carbon and high levels
of nitrogen. The power-time curves of the effluent have the typical shape of microbial growth yielding microbial growth rate
constants between 0.37 and 0.53 h−1 for about 4 and 11 h. The addition of the effluent to the soil decreases the heat of pyrolysis with time and stimulates the
heat flow rate of the microbial metabolism.
., Drochner, W. (1998.): Effect of a silage additive based on lactic acid bacteria on grass silage quality, digestibility, feed intake and milk production of dairy cows. Proceedings of the conference on nutrition of domestic animals, 30–38. p
Thermal analysis was used to deduce the mechanism of resistance to enzymatic digestion by starches and to account for the extent of resistance at different enzymolysis reaction temperatures. Thermalanalysis was also used to determine the most productive treatment temperature for exploration of the effects of heat-moisture treatment of starches on their subsequent chemical and physical behavior, including enzyme digestibility. The starches were selected according to an experimental design based on a nontraditional description of genetically varied corn starches. As a result, each functional response to heat moisture treatments of the starches adjusted to different moisture contents could be assigned to the relevant causative structural factor in the experimental design.
The aim of our studies is to check the possibilities of using proton activation analysis as a competitive method over other
analytical techniques applied for iodine determination. It is well known that long-term irradiation of biological samples
leads to their decomposition and formation of gaseous radiolysis products, which increase the pressure inside the sample container.
In case of using proton beam another problem with liquid samples appears. It is the production of 7Be via spallation reactions 16O(p, spall)7Be. The Compton effect from 7Be γ-line increases the detection limits for isotopes with low-energy γ-lines. AIC-144 cyclotron at The Niewodniczański Institute
of Nuclear Physics Polish Academy of Science can accelerate protons up to energy of 60 MeV which is sufficient for (p,5n)
reaction needed to obtain 123I (T1/2 = 13.27 h, Eγ = 159 keV, I = 83%) from stable 127I, thus the Compton effect from 7Be was the main factor perturbing the analysis. Separation and removal of 7Be is required to improve the detection limit. The paper presents a method and an example of its application to the determination
of iodine concentration in digested fragments of human thyroids obtained during surgical treatment of patients with different
types of thyroid tumor.