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Authors: Ernő Tyihák, Ágnes Móricz, Judit Mátyási, Péter Ott and József Balla

The present and more recent observations suggest that the ozone is an indispensable, endogenous molecule form, and so it can be detected and measured practically in all biological systems. There are already different indirect and direct methods for the detection and measurement of this small molecule. The endogenous formation of ozone in the biological world may open a totally new horizon, e.g., in the topics of disease resistance and cell proliferation. Special efforts and more and more efficient methods are needed for observing the endogenous reactions and functions of this very reactive key molecule.

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A new method is reported for the evaluation of non-isothermal kinetic data for various forms of the conversion function. The algorithm is based on a pseudo-inverse matrix method. A description of the algorithm and some calculation examples are presented.

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The minimum degree ordering is one of the most widely used algorithms to preorder a symmetric sparse matrix prior to numerical factorization. There are number of variants which try to reduce the computational complexity of the original algorithm while maintaining a reasonable ordering quality. An in-house finite element solver is used to test several minimum degree algorithms to find the most suitable configuration for the use in the Finite Element Method. The results obtained and their assessments are presented along with the minimum degree ordering algorithms overview.

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Authors: M. Matloobi, H. Rafii, D. Beigi, A. Khalaj and M. Kamali-Dehghan

Abstract  

This report covers optimization of radioiodination of peptides by both a direct method in which a constituent tyrosine residue is labeled and indirect method by using an iodinated derivative (SIB) of N succinimidyl 3-(tri-n-butylstannyl) benzoate (ATE) as the intermediate. Radioiodination of IgG and FMLF were performed by direct method using Chloramine-T as an oxidant but since Formyl-Methyl-Leucyl-Phenylalanine, FMLF, does not lend itself for direct radioiodination we performed labeling of FMLF by indirect method via radioiodined SIB at different pH.

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A rapid and direct method for the production of a number of carrier-free radiobrominated compounds is described, [77Br]-5-Bromo-2’-deoxyuridine is shown to be taken up by actively growing E. Coli cultures and incorporated into cellular nucleic acids.

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Phosphorus in orchard leaves (NBS SRM-1571) and spinach (SRM-1570) was determined by various substoichiometric analytical methods such as the direct method, GRASHCHENKO's method and the method of carrier amount variation. All samples were labelled with32P radioisotope. The data obtained by the method of carrier amount variation were also treated by the method of least squares instead of De VOE's method. Phosphorus concentration in orchard leaves was 0.206±0.011% by the direct method, 0.219±0.011% by GRASHCHENKO's method, 0.211±0.011% by the method of carrier amount variation and 0.207±0.007% by the method of least squares, respectively. These values agree with the value reported by NBS (0.21±0.01%). Furthermore, these concentrations obtained by various substoichiometric methods were compared with those by radioactivation reported in a prevoius paper.

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Polyclonal immunoglobulin IgG was labeled using three different methods: a direct method via 2-mercaptoethanol, an indirect one, in which a chelating group was covalently attached to the protein and the99mTc added as a glucoheptonate complex and a photoactivation method. The properties of99mTc polyclonal antibody labeled by three different methods were assessed by in vitro and in vivo studies. The ratio between inflamed thigh to normal thigh was similar and independent of the method of labeling.

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A simple and direct method has been used to obtain fairly accurate total atomic attenuation, total atomic photoelectric and total scattering cross sections by measuring the transmission factor and K X-ray fluorescence spectrum induced by 60 keV -rays for Gd, Tb, Dy and Er by employing a Si(Li) detector system in a 2 geometry set-up. All the parameters are compared with their corresponding theoretical values and a good agreement between them is obtained.

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Authors: A. Onana, S. Y. Kwankam and E. Zoue

Summary A direct method is developed for the discrete solution of Poisson's equation on a rectangle. The algorithm proposed is of the class of marching methods. The idea is to generalize the classical Cramer's method using Chebyshev matrix polynomials formalism. This results in the solution ofN independent diagonal system of linear equations in the eigenvector coordinate system. An elementary transformation to the original coordinate system is then carried out.

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A low-background -counter and a simple direct method, based on total -counting, have been developed in order to estimate the concentration of uranium at the g/g level in ordinary rock samples. The method is a relative one; international standards have been used for calibration. Only 3 g of rock powder is needed. Combining this method with -spectrometry, direct determination of238U (independently of226Ra) becomes possible near the above concentration level.

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