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The effect of free-range versus cage management system on corticosterone transfer into the eggs was studied in laying hens. Hungarian Yellow laying hens (age: 21 weeks, body weight: 2.0 ± 0.5 kg) were divided into two groups in the spring: Group I, free-range keeping (n = 15 layers, density: ≯ 0.5 bird/m2) in outdoor runs, with continuous access to a commercial layer feed; Group II, hens kept in battery cages (n = 17 layers, density: 2 birds/m2, natural light, continuous access to feed and water). Eggs were collected after a one-week adaptation period on days 2, 7 and 16. Corticosterone (CST) was extracted from homogenised egg samples using an ASE-200 Accelerated Solvent Extractor and then assayed by liquid chromatography linked with tandem mass spectrometry (LC-MS/MS) [Thermo Quest Surveyor high-performance liquid chromatography (HPLC) interfaced via Atmospheric Pressure Chemical Ionisation (APCI) ion source to Finnigan/Thermo Quest LCQ Deca MS/MS] using dexamethasone as internal standard with positive APCI ionisation. CST concentrations of whole eggs laid by free-range hens on days 2, 7 and 16 were 0.370 ± 0.218, 0.259 ± 0.066 and 0.915 ± 0.745 ng·g-1, respectively, while those of eggs laid by caged hens were 0.206 ± 0.157, 0.223 ± 0.165 and 0.184 ± 0.110 ng·g-1 at the above sampling times. It is concluded that in free-range laying hens the sharp changes of environmental weather conditions significantly increased the corticosterone content of eggs, while the environmentally controlled and closed battery cage management technology resulted in relatively uniform corticosterone concentrations in the whole eggs.

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Progress in Agricultural Engineering Sciences
Authors: Emna Ayari, Csaba Németh, Karina Ilona Hidas, Adrienn Tóth, Dávid Láng and László Friedrich

used to extend the shelf-life of eggs products and preserve their properties as coating the eggs with petroleum jelly (Vaseline), immersions in limewater and water glass ( Oladejo D. et al., 2015 ). Coating of the egg shell takes a considerable duration

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Progress in Agricultural Engineering Sciences
Authors: Emna Ayari, Csaba Németh, Karina Ilona Hidas, Adrienn Tóth, Dávid Láng and László Friedrich

used to extend the shelf-life of eggs products and preserve their properties as coating the eggs with petroleum jelly (Vaseline), immersions in limewater and water glass ( Oladejo D. et al., 2015 ). Coating of the egg shell takes a considerable duration

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MA, C.Y., SAHASRABUDHE, M.R., POSTE, L.M., HARWALKAR, V.R., CHAMBER, J.R. & O'HARA, K.P.J. (1992): Gamma irradiation of shell eggs. Internal and sensory quality, physicochemical characteristics and functional

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., Magwire, H. (1992): Eggs and egg products. - in: Vanderzant, C. & Splittoesser, D. F. (Eds), Compendium of methods for the microbiological examination of foods. American Public Health Ass., Washington D.C., pp. 857

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Ayadi, M.A., Khemakhem, M. & Belgith, H. (2008): Effect of moderate spray drying conditions on functionality of dried egg white and whole egg. J. Fd Sci. , 73 (6) E281–E287

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Angell, R. W., Hancock, J. W. (1989) Response of eggs of Heterocypris incongruens (Ostracoda) to experimental stress. J. Crustacean Biol. 9 , 381–386. Hancock J. W. Response of eggs

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Introduction Egg white (EW) is of great interest for many culinary and industrial applications. EW is used for coating, gluing, thickening and so on in pasta, desserts, etc. There is thus a great interest from the industrial

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253 257 Chandler, J.H., Musick, G.J., Fairchild, M.L. 1966. Apparatus and procedure for separation of corn rootworm eggs from soil. J. Econ. Entomol. 59 :1409

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Rombough, P. J. (1985) Initial egg weight, time to maximum alevin wet weight, and optimal ponding times for chinook salmon (Oncorhynchus tshawytscha). Can. J. Fish. Aquat. Sci. 42 , 287

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