Authors:Irma Podolak, Zbigniew Janeczko, Jan Krzek, Anna Apola, and Dorota Walusiak
A thin layer chromatographic method with densitometric UV detection at
= 285 nm has been developed for quantification of embelin in
. TLC analysis was performed on aluminumbacked silica gel 60 F
-hexane-ethyl acetate, 7 + 3 (
), as mobile phase. Under these experimental conditions the method was highly sensitive (the limit of detection was 12.5 ng) and recovery was satisfactory (from 89.45% to 92.28%). Good results obtained during validation of the method were confirmed in quantification of embelin, because high precision and accuracy were achieved.
The thermal decomposition of the complexes: [Fe(C17H24O4)1.5·2H2O]n and [UO2(C17H24O4·2H2O]n, and evaluation of kinetic parameters (E, Z andΔS) by making use of Piloyan-Novikova, Coats-Redfern and Horowitz-Metzger equations are reported. The complexes are found to decompose in three well defined steps involving random nucleation mechanism. The intermediates formed during decomposition usually undergo further decomposition without remaining stable over a considerable range of temperature.
Vidangarista is an ayurvedic polyherbal formulation officially recorded in the Ayurvedic formulary of India and used as an anthelmintic. The herbal formulation contains many plant constituents, and hence a large number of markers should be quantified to assess its quality. This paper reports the standardization of Vidangarista by HPTLC analysis for the presence of the biomarkers gallic acid and conessine. Each marker compound showed a linear relationship with an average r2 = 0.99 in the concentration range studied. The proposed method was found to be precise, specific, and accurate, with a recovery of 99–101%, and hence can be used for routine analysis of this formulation.
Authors:I Bin-Jaliah, AM Hussein, HF Sakr, and EA Eid
: Cardioprotective effect of embelin on isoproterenol-induced myocardial injury in rats: possible involvement of mitochondrial dysfunction and apoptosis . Life Sci. 107 , 59 – 67 ( 2014 ) 10.1016/j