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Singlet oxygen ( 1 O 2 )-catalyzed oxidation of water leads to a variety of oxidants (e.g. ozone, O 3 ) in host-parasite relationships as well. It can be detected indirectly in TLC or OPLC zones by use of the simple BioArena system and O 3 -eliminating molecules (e.g. d -limonene and indigo carmine) in the culture medium. It follows from these new findings that not only formaldehyde but also O 3 and related bioreactive compounds may play a crucial role in the mechanism of antibacterial activity of antibiotic-like compounds. The toxic potential of a molecule, however, originates from the ratio of the oxidants produced in the chromatographic spots.

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The present and more recent observations suggest that the ozone is an indispensable, endogenous molecule form, and so it can be detected and measured practically in all biological systems. There are already different indirect and direct methods for the detection and measurement of this small molecule. The endogenous formation of ozone in the biological world may open a totally new horizon, e.g., in the topics of disease resistance and cell proliferation. Special efforts and more and more efficient methods are needed for observing the endogenous reactions and functions of this very reactive key molecule.

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The most current in vitro and in vivo results in the BioArena system and under greenhouse conditions provide a good opportunity for a fundamental renewal of biological detections and interactions in layer liquid chromatography. The adsorbent bed in a column liquid arrangement is not suitable for biological detection because the living cells do not grow there. Contrarily, the planar adsorbent layer enables the in situ biodetection of antimicrobials and the interactions among separated compounds, cells, and further various cofactors (molecules), making the study of mechanisms of action possible. The basic elements of the time- and dose-dependent quadruple immune response of plants to pathogens in relation to the function and reactions of formaldehyde and its reaction products (mainly endogenous ozone) were demonstrated. This finding opens a new horizon in the field of disease resistance in plants and perhaps in general in the biological world. These results give a good basis and possibility for studying and understanding the unique high-dilution phenomena as well, and at that time, they promise the elimination of century contradictions in this field.

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