Authors:Haiqun Cao, Yongde Yue, Rimao Hua, Feng Tang, Rong Zhang, Wei Fan, Haiyan Chen, and Yong Hua
A simple, rapid, and sensitive high-performance thin-layer chromatographic method has been developed and validated for analysis of residues of imidacloprid, fenitrothion, and parathion in Chinese cabbage. The sample was extracted by sonication in an ultrasonic water bath with acetone-petroleum ether, 5:3 (
), and the extract was directly applied, as bands, to glass-backed silica gel 60F
HPTLC plates. The plates were developed with hexane-acetone, 7 + 3 (
), in an unsaturated glass twin-trough Camag chamber. Evaluation of the developed HPTLC plates was performed densitometrically with a Camag TLC Scanner 3 controlled by an external PC running Wincats software (Version 1.1.2). The results indicated that the detection limits of imidacloprid (
= 0.10), fenitrothion (
= 0.59), and parathion (
= 0.70) were 5.0 × 10
g, 2.0 × 10
g, 1.0 × 10
g, respectively. Recoveries of the pesticides from Chinese cabbage by use of this analytical method were 80.04–85.22%, and
were 4.18–13.15%. The precision and accuracy of the method were generally fit for analysis of pesticide residues in Chinese cabbage.
Authors:Wei Fan, Yongde Yue, Feng Tang, Haiqun Cao, Jing Wang, and Xi Yao
A high-performance thin-layer chromatographic (HPTLC) method for simultaneous analysis of temephos and fenitrothion in green tea has been established and validated. The sample was extracted with ethyl acetate by mechanical vibration at room temperature and the extract was cleaned by use of an SPE tandem column, before elution with dichloromethane. The extract was applied as bands to glassbacked silica gel 60F254 HPTLC plates. The plates were developed with acetone-hexane 3+7 (v/v), in an unsaturated glass twin-trough chamber. The developed HPTLC plates were evaluated densitometrically. The detection limits of temephos (RF 0.55) and fenitrothion (RF 0.69) were 20 and 10 ng, respectively. Recovery from green tea of the two pesticides at levels of 0.2 to 4 mg kg−1 was 80–107% with relative standard deviations of 4.4–20.2%. The precision and accuracy of the method were generally fit for analysis of temephos and fenitrothion residues in green tea.
Thin-layer chromatography in combination with fiber optical (diode array) scanning densitometry has been used for identification of fenitrothion in apples and fresh apple juice. The technique enabled parallel recording of chromatograms and in-situ UV-visible spectrometry in the range
= 191–612 nm.
Authors:A. Sheikhi-Garjan, A. Hosseini-Gharalari, M. Mahjob, M. Rashid, Q. Sabahi, M. Safari, F. Jalilyan, and R. Arbabtafti
Sunn pest, Eurygaster integriceps, is a key pest of wheat and barley in Iran. At present, infested areas are treated with ground sprayers. A survey was conducted to evaluate the efficiency and calibration accuracy of ground hydraulic sprayers in Iran. The results showed that the motorized lance sprayer and manually operated sprayer were the most conventional sprayers, and fenitrothion EC 50% and deltamethrin EC 2.5% were the conventional insecticides against sunn pest in Kermanshah province. The mean efficacy percentage was 88% and 35% against nymphs and overwintered adults, respectively. In motorized lance sprayers, there were 5% and 90% overdosage of fenitrothion and deltamethrin, respectively; while, in manually carried sprayers, there were 5% and 25% overdosage of fenitrothion and deltamethrin, respectively. Applied dosages were 5-20% and 25-90% more than recommended dosages of fenitrothion and deltamethrin, respectively. Errors in effectiveness and applied dosage were higher in motorized lance sprayer than manually carried sprayers.
Authors:T. Varga, I. Hlubik, L. Várnagy, P. Budai, and E. Molnár
The purpose of this work was to determine the individual and combined effects of insecticide Sumithion 50 EC (50% fenitrothion) and herbicide Fusilade S (12.5% fluazifop-P-butyl) on the development of pheasant embryos. Eggs were treated by injection of various concentrations of pesticides into the air space on day 12 of incubation. Pathological examination of embryos was carried out on day 23 of the hatching period. Mortality rate, body weight data and morphological alterations were evaluated after the macroscopic examination. The skeletal staining method was used to detect deformities. The two pesticides used in combination moderated the toxic/teratogenic effects of individual treatment.
Authors:Mingyu Zhao, Ercheng Zhao, Junxue Wu, Yuqi Li, and Baotong Li
successfully established. Materials and methods Reagents Individual standard solutions (100 μg mL −1 ) prepared in acetone (HPLC grade) of five organophosphorus pesticides (ethoprophos, diazinon, fenitrothion, chlorpyrifos, and profenofos) were obtained from
. Abstract Book of the SETAC Europe 15th Annual Meeting, May 22–26 2005, Lille, France, p. 230.
Babczynska, A., Migula, P. (2002) Cadmium-fenitrothion interaction in the spider