Authors:Wojciech Markowski, Katarzyna Czapińska, Genowefa Misztal and Łukasz Komsta
Conditions have been established for separation of six fibrate drugs by automatic multiple development (AMD). The best system was selected by using results from systematic investigation of relationships between
values and mobile-phase composition. The drugs were separated by multiple development on diol plates with tetrahydrofuran-hexane as mobile phase. The results of multiple development analysis predicted by computer simulation were compared with experimental results.
Authors:Genowefa Misztal, Łukasz Komsta and Dorota Cichecka
values and mobile phase composition have been examined for six fibrate-type drugs — bezafibrate, ciprofibrate, clofibrate, clofibric acid, fenofibrate, and gemfibrozil. They were separated in horizontal chambers on RP18 plates by use of mobile phases containing phosphate buffer and different amounts of six modifiers — acetone, acetonitrile, dioxane, isopropanol, methanol, and tetrahydrofuran. Plates were visualized under UV irradiation at
= 254 nm, and scanned with a densitometer in multi-wavelength scan mode. Optimum modifier concentrations were also investigated on RP8 and CN plates for comparison. The linearity of relationships between
and modifier volume fraction, molar fraction, and the logarithm of the molar fraction was calculated. Most results fitted the
> 0.95; for almost half
> 0.99. Separation of all the drugs was achieved on RP18 plates with mobile phase containing 70% dioxane in pH 7.60 phosphate buffer.
values and mobile phase composition have been determined for the six antihyperlipidemic agents-bezafibrate, ciprofibrate, clofibrate, clofibric acid, fenofibrate, and gemfibrozil. The drugs were separated on silica gel, CN, and Diol plates using mobile phases containing
-hexane as weakly polar diluent and five polar modifiers: acetone, dioxane, ethyl methyl ketone, ethyl acetate, and tetrahydrofuran. The optimum mobile phases were also investigated on alumina, NH
, and polyamide phases for comparison. The linearity of relationships between
and modifier volume fraction, molar fraction, and logarithm of molar fraction was calculated. Plates were developed in horizontal chambers, visualized under UV irradiation at
= 254 nm, and scanned with a densitometer in the multi-wavelength scan mode. Most results fitted the
> 0.9; for approximately half
> 0.99. Separation of all the drugs was achieved on Diol plates with mobile phases containing 20–30% of each modifier in
-hexane, and with hexane-acetone, 9 + 1, on CN plates. Five drugs were separated using the same mobile phases on silica gel. The best separation was obtained on Diol plates with tetrahydrofuran-hexane, 2 + 8, as mobile phase.
Authors:Pankaj Jain, Ramesh Roop Rai, Harsh Udawat, Sandeep Nijhawan and Amit Mathur
The combination of heparin and insulin is a newer but not well-studied modality for the treatment of hypertriglyceridemia-induced acute pancreatitis. We report three patients of hypertriglyceridemia induced mild acute pancreatitis. Two patients had evidence of acute pancreatitis on abdominal CECT but their amylase and lipase levels were less than three times the upper limit of normal. Two patients were treated with heparin and insulin followed by fibrate, and the third was treated conservatively with fibrate only. The patients treated with insulin and heparin showed a rapid fall in triglyceride level (52%) on day one as compared to the patient treated conservatively (17.5%) with marked improvement in the pain score as assessed by the visual analogue scale. All the patients were maintained on fibrates to keep a level of triglycerides less than 200 mg/dL and were asymptomatic at 15, 27 and 40 months of follow-up.
Tenenbaum, A., Fisman, E. Z.: Fibrates are an essential part of
modern anti-dyslipidemic arsenal: spotlight on atherogenic dyslipidemia and
Authors:Łukasz Komsta, Wojciech Markowski and Genowefa Misztal
The retention factor
is used in several criteria generally known as chromatographic response functions (CRF). In TLC and HPTLC most of these are based on differences between the retention factors of two substances, which are summed or multiplied. There are also other functions, for example the multispot response function (
) which enables the quality of a separation to be estimated. Although good CRF criteria should have well-defined distribution and range, current criteria based on the differences do not satisfy this requirement. Only
has a clearly defined range (0 to 1), but its distribution is unstable. In this paper two new independent coefficients:
(retention uniformity) and
(retention distance) are proposed; these always have values within the range 0 to 1 and stable density, irrespective of the number of compounds separated. Their reliable mathematical properties have been tested in wide range by Monte-Carlo simulations. An example is given of their use in the separation of fibrate-type antihyperlipidemic drugs by normal and reversed-phase TLC (114 systems).
Authors:Tatjána Ábel, Anna Blázovics, Márta Kemény and Gabriella Lengyel
A várandósság ideje alatt a lipoproteinszintek változása élettani folyamat. Hyperlipoproteinaemiás betegeknek a terhesség tervezésétől az újszülött szoptatásának befejezéséig nem ajánlott statinok, fibrátok szedése az esetleges teratogén hatások miatt. Terhesség során a hypertriglyceridaemia ritkán akut pancreatitishez vezethet. Az akut pancreatitis kezelése megegyezik a nem várandós betegek kezelésével. További nagy betegszámot felölelő vizsgálatok szükségesek annak megítéléséhez, hogy a terhességben kialakult vérzsírszintváltozások milyen mértékű cardiovascularis kockázatot jelentenek a későbbi évek során. Orv. Hetil., 2011, 152, 753–757.