Authors:Sweta Jha, Ashok Bhagwat, and Nancy Pandita
The γ-amino butyric acid (GABA) is the main inhibitory neurotransmitter system in the brain. Either imbalance in the extreme can result in several mental diseases like schizophrenia, cerebral stroke, temporal lobe epilepsy (TLE), Parkinson’s disease (PD), Huntington’s disease (HD), and anxiety disorders even death. Measurement of GABA in tissue will help to elucidate the metabolic role and diagnostic value. Various analytical techniques are employed to estimate GABA in biological samples but the experimental procedure and tedious techniques are required. The present study demonstrates a simple, feasible, and cost-effective high-performance thinlayer chromatography (HPTLC) method for estimation of GABA in mice brain tissue. The method was validated in terms of specificity, linearity, and detection limit. The precision was done for inter-day, intra-day, instrumental, and reproducibility. Accuracy was checked by recovery study. The results indicate that this method is fast, sensitive, and suitable for quantitative assessment.
., Bossi, L., Morselli, P. L., Munari, C., Rougier, M., Loiseau, H. (1986) Alterations of GABA-mediated synaptic transmission in human epilepsy. In: Delgado-Escueta, A. V., Ward, A. A., Woodbury, D. M., Porter, R. J. (eds) Basic Mechanisms of the
Authors:E.E. Sage, Y.X. Chang, R. Wickneswari, and M.M. Mackeen
GABA is a non-protein amino acid that can be naturally found in the brain ( Roberts & Frankel, 1950 ) and other non-neuronal tissues such as ovarian tissue ( Del Rio & Caballero, 1980 ) in mammals. It can also be found in plants
Authors:K. K. Akula, B. Chandrasekaran, M. Kaur, and S. K. Kulkarni
A new, rapid, and specific reversed phase high-performance liquid chromatographic (RP-HPLC) method involving precolumn derivatization with benzoyl chloride was developed and validated for the estimation of γ-aminobutyric acid (GABA) in rat brain tissue preparations. The derivatization product of GABA was identified by melting point, infrared, and proton nuclear magnetic resonance (1H NMR) spectroscopy to be n-benzoyl GABA. Various parameters which influenced derivatization and elusion were optimized. The chromatographic system consisted of C-18 column with ultraviolet (UV)—photodiode array detection ranging from 210 to 400 nm. Elution with an isocratic mobile phase consisting of 0.025 M disodium hydrogen phosphate buffer—methanol (65:35, v/v; pH 6) at a flow rate of 1 mL min−1 yielded sharp and specific peak of n-benzoyl GABA within 7 min. The method was validated with respect to the linearity, accuracy, precision, sensitivity, selectivity, and stability, wherein the benzoyl derivative of GABA showed stability for 2 months. The lower limit of detection was 0.5 nmol L−1. This novel derivatization procedure for the estimation of GABA with benzoyl chloride was also applied for rat brain tissue preparations that gave highly specific peak and good component recovery. The results show that the method for the determination of GABA by benzoylation using RP-HPLC has good linearity, accuracy, precision, sensitivity, and specificity and is simple and economical to perform.
Authors:B. Czéh, Hajnalka Ábrahám, Siroun Tahtakran, Carolyn Houser, and L. Seress
Aika, Y., Ren, J. Q., Kosaka, K., Kosaka, T. (1994) Quantitative analysis of GABA-likeimmunoreactive and parvalbumine-containing neurons in the CA1 region of the rat hippocampus using a stereological method, the disector. Exp. Brain Res. 99, 267