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The aims of the study were the optimisation of genomic in situ hybridization (GISH) and the use of a GAA satellite sequences as a fluorescence in situ hybridization (FISH) probe to discriminate Aegilops biuncialis and wheat chromosomes. The application of genomic DNA from the diploid progenitors, Ae. umbellulata and Ae. comosa , as a probe with a blocking ratio of 1:200 resulted in the clear visualization of Ae. biuncialis chromosomes. Based on the strong hybridization signals of the PCR-amplified GAA satellite sequences it was possible to identify chromosomes 1U, 2U, 4U and 5U of Ae. umbellulata . The improved GISH and GAA idiogram presented here allow the Ae. biuncialis chromatin to be traced in more detail during chromosome-mediated gene transfer to wheat.

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Wild barley, Hordeum marinum subsp. gussoneanum (2n = 28) is a valuable source of genes that determine resistance to abiotic stresses. These resistance traits might be transferred to wheat due to the crossability of wild barley with bread wheat. The availability of reliable and rapid methods for the identification of H. marinum subsp. gussoneanum chromatin in a wheat background would facilitate the development of introgression wheat genotypes. For this purpose, we evaluated the applicability of eighty-seven H. vulgare EST markers for studying bread wheat – H. marinum subsp. gussoneanum substitution and addition lines. Of all of the markers studied, forty-three (49%) were amplified in H. marinum ssp. gussoneanum and wheat introgression lines. The identification of wild barley chromosomes using EST markers confirmed the GISH and C-banding data. Thus, it was established that the H. vulgare EST markers can be successfully used to identify the chromosomes of the H. marinum subsp. gussoneanum in introgression lines of wheat.

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respective homologues in a wheat-rye hybrid as revealed by GISH. Cereal Res. Comm. 28 :41–48. Molnár-Láng M. Frequency of pairing between the 1B/1R translocation and its respective

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6075 6080 Cseh, A., Kruppa, K., Molnár, I., Rakszegi, M., Dolezel, J., Molnár-Láng, M. 2011. Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH

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In order to investigate the origin of two 4B-5R wheat-rye translocations a number of microsatellite markers were considered, (a) which were specific for the rye chromosome arm 5RL and (b) which should enable precise characterisation for the gene pool of a ‘Cornell’ wheat derivative. Seven out of eight markers revealed amplification fragments with the rye control, while the marker WMS0186 amplified only a PCR product with the appropriate chromosome arm of the wheat control. The heterogeneity of the DNA fragment patterns among five wheat-rye translocation lines confirms different wheat backgrounds of the American, Danish/UK, Hungarian, and Swedish sources of the 4B-5R translocations. The homogeneous DNA marker patterns of ‘Viking-hairy neck’ and ‘Cornell Sel. 82a1-2-4-7’ are particularly clear. The corresponding molecular markers together with the cytological data, genomic in situ hybridisation, the physiological investigations, and the historical review support the hypothesis of a common origin of ‘Viking-hairy neck’ and ‘Cornell Sel. 82a1-2-4-7’. The importance of the results for practical breeding and for introgression experiments is discussed.

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hibridizációval (GISH). (Molecular cytogenetic analysis of a new wheat-rye hybrid with C-banding and genomic in situ hybridization (GISH).) Növénytermelés , 3 , 253-260. Új búza-rozs amfiploid molekuláris genetikai elemzése C

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amphiploids of wheat- Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor. Appl. Genet. 109: 1070–1076. Liu Z. Genomic constitution

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research and control in China. BGRI 2010 Technical Workshop, St Petersburg, Russia, pp. 1–21. Kishii, M., Wang, R.R.C., Tsujimoto, H. 2005. GISH analysis revealed new aspect of genomic constitution of Thinopyrum

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the development of wheat/barley disomic additions in winter wheat varieties, and identification using molecular cytogenetic techniques (C-banding, GISH)]. Növénytermelés , 52 , 3–13. Molnár-Láng M

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. Han , F.P. , Liu , B. , Fedak , G. , Liu , Z.H. 2004 . Genomic constitution and variation in ?ve partial amphiploids of wheat-Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis . Theor

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