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Authors:Hagen Frickmann, P. Gawlik, S. Crusius, and A. Podbielski
Struelens MJ, Deplano A, Godard C, Maes N, Serruys E: Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gelelectrophoresis. J Clin
Authors:E. M. Faergestad, M. B. Rye, S. Nhek, K. Hollung, and H. Grove
Chemometrics involves strategies to analyse multivariate data using interdisciplinary approaches aiming to extract relevant information from complex data. Chemometric strategies comprise both the pre-processing of the data, where the choice of methodology is domain-specific, and analysis of the resulting data after preprocessing using multivariate methodology. Although use of multivariate data analysis for gel electrophoresis images has increased substantially in the last decade, its use is still much less frequent than use of univariate approaches. Considering the complexity of the electrophoresis gel images and the multivariate nature of the proteome, applying multivariate data analysis for gel electrophoresis images gives information which is otherwise lost. This paper is written as a review and guideline of chemometric strategies used for analysis of gel electrophoresis images. The multivariate data analyses described are, however, also relevant for other proteome data, for example mass spectrometry, and for functional genomics in general.
Authors:G. Weseloh, M. Kühbacher, H. Bertelsmann, M. Özaslan, A. Kyriakopoulos, and A. Knöchel
For the analysis of metal-containing proteins, sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been
synchrotron radiation X-ray fluorescence (SRXRF). In a pilot study the applicability of this combined method was tested in
the analysis of metalloaded
apoazurin and of the selenoproteins in rat testis homogenate. It was shown that it can be applied in the determination of
the major stable
binding forms of trace elements. After further improvement of the limits of detection the method will allow the analysis of
proteins present in the samples at low concentrations.
Authors:Eleonóra Fodor, E. Dósa, Á. Nagy, E. Nagy, and L. Ferenczy
In the present study, 16 women with recurrent vulvovaginal candidiasis (RVVC) due to Candida albicans and Candida (Torulopsis) glabrata were followed for a period of 4 to 12 months, and 36 vaginal isolates were evaluted by pulsed-field gel electrophoresis (PFGE). Eleven women were infected by C. albicans and5 by C. glabrata.Three electrophoretic karyotypes of C. albicans and 3 of C. glabrata were identified throughout the follow-up. All patients but one was infected with the same karyotype of C. albicans or C. glabrata during the follow-up period.Two different karyotypes of C. glabrata were identified in one patient in the course of 12 months. The results confirmed the diversity of the karyotypes of C. albicans and C. glabrata causing vulvovaginitis and demonstrated the persistence of colonization with the same strain over different periods of time despite therapy (15/16 women).