Biological Development Co., Ltd. (purity: ∼99%). Saline (medical), Glucose (medical).
Equipment and conditions
Thermoanalyzer Systems (American Thermoanalyzer Companies Inc.) were used for determining the DSC–TG curves of
Authors:Carmen Romero, Alberto Albis, José Lozano, and Javier Sancho
Thermal stability of bovine α-lactalbumin in buffer and dilute aqueous solutions of erythritol, xylitol, sorbitol, inositol
and glucose was evaluated by fluorescence spectroscopy and circular dichroism. Results show that at the selected conditions,
the transition is reversible and is well described by a two-state model. At low concentration the cosolutes do not show a
structure stabilizing effect, and some of them even destabilize the protein. At higher concentration, all of them stabilize
the native protein conformation; however, the extent of stabilization is lower than the effect shown with other proteins,
presumably due to the lactalbumin incomplete unfolding.
The catalytic properties of a novel MFI-type zeolite with different SiO2/Al2O3 ratio in the dehydration of glucose to levulinic acid (LA) were investigated in this work. The results demonstrate the strength
of acidic sites and the mesoporosity of the zeolites have significant effects on LA formation.
Authors:Robin Steudtner, Thuro Arnold, Gerhard Geipel, and Gert Bernhard
Cryogenic techniques are currently used in scanning tunnelling microscopy (STM) and single molecule spectroscopy. Recently
such cryogenic devices have also been adapted to time resolved laser-induced fluorescence spectroscopy (TRLFS) systems applied
to uranium(VI). In our study, we interpret TRLFS results obtained for the uranyl(VI) glucose system at room temperature (RT)
and under cryogenic conditions of 153 K (cryo-TRLFS). A uranyl(VI) glucose complex was only identified by cryo-TRLFS measurements
at pH 5 and not by RT measurements. The uranyl(VI) glucose complex was characterized by five emission bands at 499.0, 512.1,
525.2, 541.7, and 559.3 nm and a fluorescence lifetime of 20.9 ± 2.9 μs. The uranyl(VI) glucose complex formation constant
was calculated for the first time to be logßI=0.1 M = 15.25 ± 0.96. Cryo-TRLFS investigation opens up new possibilities for the determination of complex formation constants
since interfering quenching effects often encounter at RT are suppressed by measurements at cryogenic conditions.
Authors:Nevena Popovic, Bernard Fried, and Joseph Sherma
Biomphalaria glabrata snails were infected with Schistosoma mansoni and maintained at different dilutions of artificial ocean water for up to 4 weeks. Glucose and maltose concentration of the digestive gland-gonad complex were analyzed by high-performance thin-layer chromatography at different stages of the infection. B. glabrata snails were divided into three experimental groups: Group A, snails with early prepatent infection (10 days post-infection); Group B, snails with late prepatent infection (22 days post-infection); and Group C, snails with patent infection (45 days post-infection). Infected snails in A were maintained at different salinities for 2 weeks and then necropsied, and their two main simple sugars, i.e., glucose and maltose, were analyzed. Groups B and C contained two subgroups: the first subgoups were analyzed after 2 weeks, and the second after 4 weeks. Controls for these experiments were maintained identically in either deionized water or artificial spring water. Maltose and glucose were extracted from the digestive gland-gonad complex in ethanol-water (70:30). 1-Butanol-glacial acetic acid-diethyl ether-deionized water (27:18:5:3) mobile phase was used to separate sugars on EMD Millipore silica gel preadsorbent plates. Sugars were detected using α-naphthol-sulfuric acid reagent and quantified with a CAMAG TLC Scanner 3 at 515 nm. The obtained data were compared using analysis of variance (ANOVA) single factor statistical analysis. Statistical differences were not found in any sugars in Group A snails. For glucose, a significant difference was found after 4 weeks in both B and C snails. For maltose, a significant difference was found after 4 weeks in B snails and after 2 weeks in C snails. Different salinity levels affect the maltose and glucose concentrations of adult B. glabrata snails infected with S. mansoni.
Authors:Teréz Márián, L. Balkay, I. Fekete, Zs. Lengyel, and G. Veress
Shawver, L. K., Olson, S. A., White, M. K., Weber, M. J. (1987) Degradation and biosynthesis of the glucose transporter protein in chicken embryo fibroblasts transformed by the src oncogene. Mol. Cell Biol. 7
Authors:Magda Alania, Varya Dyakonova, and D. A. Sakharov
Ruibal, C., Soengas, J. L., Aldegunde, M. (2002) Brain serotonin and the control of food intake in rainbow trout ( Oncorhynchus mykiss ): effects of changes in plasma glucose levels. J. Comp. Physiol. 188A , 479
Authors:Gábor Marics, Levente Koncz, Anna Körner, Borbála Mikos, and Péter Tóth-Heyn
Norhammar, A. M., Rydén, L., Malmberg, K.: Admission plasma glucose. Independent risk factor for long-term prognosis after myocardial infarction even in nondiabetic patients. Diabetes Care, 1999, 22 , 1827