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A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 F254S HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at R F = 0.63 ± 0.02 and R F = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng band−1, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug.

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A simple and rapid high-performance thin-layer chromatographic (HPTLC) method has been established for the analysis of rutin, rosmarinic acid, and glycyrrhizin, three active compounds from the decoction of Abnormal Savda Munziq (ASMq) prescription. The method employed silica gel GF254 thin-layer plates as the stationary phase, with ethyl acetate-formic acid-acetic acid-water (15:1:1:1.5, ½/½) as the mobile phase. Following development, plates were observed under ultraviolet (UV) light at 250 nm. The method was validated for linearity, precision, robustness, recovery, limit of detection (LOD), and limit of quantification (LOQ). Linear regression analysis data for the calibration plots showed good linear relationships (r) from 0.9992 to 0.9993. Recovery of the three compounds was between 97.86 and 100.77%. LOD and LOQ were in the range of 33.30–38.17 ng and 110.80–127.26 ng for the three compounds, respectively. The method is rapid, simple, effective, and easy-to-use for ASMq to perform routine quality control analysis and stability studies in commercial preparations.

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Summary

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of glycyrrhizin, formononetin, glycyrrhetinic acid, liquiritin, isoliquiritigenin, and licochalcone A in licorice. An Eclipse Plus C18 column (I.D. 4.6 × 100 mm, 3.5 μm particle size; Agilent) was used in the analysis. Electrospray ionization (ESI)-tandem interface in the negative mode was performed, and multiple reaction monitoring (MRM) was employed with the precursor multiple reaction monitoring production combination for the determination of six analytes. The average recoveries ranged from 98.30% to 100.13% with relative standard deviations (RSDs) ≤ 1.95%, and limits of detection (LODs) ranged from 2.1 to 3.6 pg. The applicability of this analytical approach was confirmed by the successful analysis of six samples. The results indicated that the established method was validated, sensitive, and reliable for the determination of six analytes in licorice.

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://www.eucast.org Sasakia K, Yonebayashia S, Yoshidaa M, Shimizua K, Aotsukaa T, Takayamab K: Improvement in the bioavailability of poorly absorbed glycyrrhizin via various nonvascular administration routes in rats. Int J Pharm 265(1–2), 95–102 (2003

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Abe, M., Akbar, F., Hasebe, A., Horiike, N., Onji, M. (2003) Glycyrrhizin enhances interleukin-10 production by liver dendritic cells in mice with hepatitis. J. Gastroenterol. 38 , 962

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berberine, baicalein and glycyrrhizin in medicinal plants . Anal. Chim. Acta , 482 , 81 – 89 . O U , B.X. , H AMPSCH -W OODILL , M. & P RIOR , R.L. ( 2001 ): Development and

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, Burdock GA: Risk and safety assessment on the consumption of Licorice root (Glycyrrhiza sp.), its extract and powder as a foodingredient, with emphasis on the pharmacology and toxicology of glycyrrhizin. Regul. Toxicol. Pharmacol. 46, 167–192 (2006

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Acta Chromatographica
Authors: Filip Šibul, Dejan Orčić, Sanja Berežni, Goran Anačkov and Neda Mimica-Dukić

, emodin, aloe-emodin, cis, trans –abscisic acid, isoliquiritigenin, ellagic acid, glycyrrhizin, 18 β -glycyrrhetinic acid, 4-hydroxyphenylacetic acid, formononetin, isoscopoletin, ursolic acid, and resveratrol. Plant Material and

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Acta Chromatographica
Authors: Qinghua Weng, Lianguo Chen, Luxin Ye, Xiaojie Lu, Zheng Yu, Congcong Wen, Yichuan Chen and Gang Huang

phlegm, and the treatment of asthma, pain, fever cough, and stomach ulcer in the Chinese Pharmacopoeia [ 1 ]. Previous investigations have shown that glycyrrhizin and its derivatives in licorice were the main active components responsible for the hepatic

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) 36. Madhavadas S , Subramanian S : Combination of Spirulina with glycyrrhizin

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