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Growth, cell viability and heat shock proteins (HSPs) in Bezostaya-1, Çukurova-86 and Diyarbakır-81 cultivated wheat cultivars and three Aegilops species were investigated. Etiolated seedlings were exposed to 23 °C, 32 °C, 35 °C, 37 °C and 38 °C for 24 h, and 35 °C (24 h) → 50 °C(1 h) and 37 °C (24 h) → (50 °C (1 h). At the end of recovery growth periods, the shoot lengths of the genotypes generally decreased significantly at 35, 37 and 38 °C. The acquired thermal tolerance (ATT) in intact seedlings was over 50% at 35 °C → 50 °C and 37 °C → 50 °C, but in cell viability test it ranged from 2.75% (Ae. triuncialis) to 32.87 (Bezostaya-1) at 35 °C, and from 2.82% (Ae. triuncialis) to 37.82 (Bezostaya-1) at 37 °C. Ae. triuncialis was most sensitive genotype in both ATT determination. In electrophoretic profiles of proteins, while some HSPs were newly synthesized, some normal cellular proteins disappeared at 37 °C and 37 °C → 50 °C compared to 23 °C. The number of low molecular weight (LMW) HSPs were more than intermediate- (IMW) and high- (HMW) HSPs. The genotypes had both common (12 HSPs between at least two genotypes) and genotype-specific (33 HSPs) LMW HSPs. The common HSP of 19.8 kDa (pI 6.5) was synthesized in Bezostaya-1, Çukurova-86, Diyarbakır-81, Ae. biuncialis and Ae. umbellulata. Bezostaya-1 is the only genotype that synthesized 12 IMW and 2 HMW HSPs at 37 °C → 50 °C. Ae. triuncialis had only two common LMW HSPs [22.1 (pI 7.1) and 24.2 kDa (pI 6.5)].
Zero control 23.6 h 50.8 k 28.6 i 35.3 k 4.1 h 10.2 k 1/2NP control 30.1 f
