Authors:Eike Reich, Anne Blatter, Ruth Jorns, Matthias Kreuter, and Kathrin Thiekötter
, Qualitative and Quantitative Analysis of Herbals and Herbal Medicinal Products (HMP) by HPTLC. In:
(Ed.) Proc. Int. Symp. Planar Separations — Planar Chromatography 2000, Lillafüred, pp. 45
An HPTLC method, using an internal standard, for analysis of colchicine in a pharmaceutical formulation, has been established and validated. The analyte and internal standard were separated on aluminum plates precoated with silica gel 60 F
; the mobile phase was ethyl acetate-acetonitrile-water-formic acid 8.0:1.0:0.5:0.5 (
). Quantification was by densitometric scanning at 358 nm. Response was a linear function of colchicine concentration in the range 5 to 35 μg mL
. The limits of detection and quantification for colchicine were 1 and 5 μg mL
, respectively. Average recovery of colchicine was 100.48%, which showed the method was free from interference from excipients present in the formulation. The established method enabled accurate, precise, and rapid analysis of colchicine in the pharmaceutical formulation.
Authors:Shimei Ge, Feng Tang, Yongde Yue, Rimao Hua, and Rong Zhang
Residues of three pyrethroids in spiked vegetables have been analyzed by HPTLC with two mobile phases in a twin-trough chamber and in horizontal chambers. The spots were detected at
= 203 nm and the greatest detection sensitivity for the three pyrethroids was 10 ng. Recovery after fortification at 0.5–5.0 mg kg
was from 70.20 to 108.5%. The relative standard deviation was 1.59–27.94%.
Authors:Shrishailappa Badami, Mahesh Gupta, Noble Mathew, Subramania Meyyanathan, Bhojraj Suresh, and David Bendell
Among the complex mixture of biologically active compounds in the bark of
, a plant used in folklore, lupeol, a constituent of the bark, has been used as an analytical marker indicative of the quality of the plant. A sensitive and reliable quantitative high-performance thin-layer chromatographic method has been developed for the determination of lupeol from
. Chloroform extracts of bark from five different sources were used for HPTLC on silica gel with benzene-ethyl acetate, 95 + 5, as mobile phase. Under these conditions the
of lupeol was 0.40. The calibration plot was linear in the range 0.5 to 1.5 μg lupeol and the correlation coefficient, 0.999, was indicative of good linear dependence of peak area on concentration. The mean assay of lupeol was 2.902 ± 0.243 mg g
bark. The method enables reliable quantification of lupeol and good resolution and separation of lupeol from other constituents of
. To ascertain the purity of the peak from the test sample its in-situ reflectance spectrum was compared with that from standard lupeol; clear superimposibility indicated the purity of the peaks. Recovery values from 98.00 to 99.78% showed the reliability and reproducibility of the method were excellent. The HPTLC method proposed for quantitative monitoring of lupeol in
is rapid, simple, and accurate and can be used for routine quality testing.